Pressurized CO2 as a carboxylating agent for the biocatalytic: Ortho -carboxylation of resorcinol

Katharina Plasch; Gerhard Hofer; Walter Keller; Sam Hay; Derren J. Heyes; Alexander Dennig; Silvia M. Glueck; Kurt Faber

doi:10.1039/c8gc00008e

Pressurized CO₂ as a carboxylating agent for the biocatalytic: Ortho -carboxylation of resorcinol

Katharina Plasch, Gerhard Hofer, Walter Keller, Sam Hay, Derren J. Heyes, Alexander Dennig, Silvia M. Glueck^*, Kurt Faber

^*Corresponding author for this work

Institute of Biotechnology and Biochemical Engineering (6510)

Research output: Contribution to journal › Article › peer-review

Abstract

The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO₂ (∼30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

Original language	English
Pages (from-to)	1754-1759
Number of pages	6
Journal	Green Chemistry
Volume	20
Issue number	8
DOIs	https://doi.org/10.1039/c8gc00008e
Publication status	Published - 1 Jan 2018

ASJC Scopus subject areas

Environmental Chemistry
Pollution

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title = "Pressurized CO2 as a carboxylating agent for the biocatalytic: Ortho -carboxylation of resorcinol",

abstract = "The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (∼30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.",

author = "Katharina Plasch and Gerhard Hofer and Walter Keller and Sam Hay and Heyes, {Derren J.} and Alexander Dennig and Glueck, {Silvia M.} and Kurt Faber",

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T2 - Ortho -carboxylation of resorcinol

AU - Plasch, Katharina

AU - Hofer, Gerhard

AU - Keller, Walter

AU - Hay, Sam

AU - Heyes, Derren J.

AU - Dennig, Alexander

AU - Glueck, Silvia M.

AU - Faber, Kurt

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (∼30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

AB - The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (∼30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

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U2 - 10.1039/c8gc00008e

DO - 10.1039/c8gc00008e

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JO - Green Chemistry

JF - Green Chemistry

IS - 8

ER -

Pressurized CO₂ as a carboxylating agent for the biocatalytic: Ortho -carboxylation of resorcinol

Abstract

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