Pressurized CO2 as a carboxylating agent for the biocatalytic: Ortho -carboxylation of resorcinol

Katharina Plasch; Gerhard Hofer; Walter Keller; Sam Hay; Derren J. Heyes; Alexander Dennig; Silvia M. Glueck; Kurt Faber

doi:10.1039/c8gc00008e

Pressurized CO₂ as a carboxylating agent for the biocatalytic: Ortho -carboxylation of resorcinol

Katharina Plasch, Gerhard Hofer, Walter Keller, Sam Hay, Derren J. Heyes, Alexander Dennig, Silvia M. Glueck^*, Kurt Faber

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Biotechnologie und Bioprozesstechnik (6510)

Publikation: Beitrag in einer Fachzeitschrift › Artikel › Begutachtung

Abstract

The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO₂ (∼30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

Originalsprache	englisch
Seiten (von - bis)	1754-1759
Seitenumfang	6
Fachzeitschrift	Green Chemistry
Jahrgang	20
Ausgabenummer	8
DOIs	https://doi.org/10.1039/c8gc00008e
Publikationsstatus	Veröffentlicht - 1 Jan. 2018

ASJC Scopus subject areas

Umweltchemie
Umweltverschmutzung

Zugriff auf Dokument

10.1039/c8gc00008e

Andere Dateien und Links

http://www.scopus.com/inward/record.url?scp=85045954923&partnerID=8YFLogxK

Dieses zitieren

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abstract = "The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (∼30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.",

author = "Katharina Plasch and Gerhard Hofer and Walter Keller and Sam Hay and Heyes, {Derren J.} and Alexander Dennig and Glueck, {Silvia M.} and Kurt Faber",

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AU - Hay, Sam

AU - Heyes, Derren J.

AU - Dennig, Alexander

AU - Glueck, Silvia M.

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AB - The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (∼30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

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