Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator

Leen Assil Companioni, Sandy Schmidt, Petra Heidinger, Helmut Schwab, Robert Kourist

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The coupling of recombinantly expressed oxidoreductases to endogenous hydrogenases for cofactor‐recycling permits the omission of organic cosubstrates as sacrificial electron donors in whole‐cell biotransformations. This increases atom efficiency and simplifies the reaction. We have expressed a recombinant ene‐reductase in the hydrogen‐oxidizing proteobacterium Cupriavidus necator H16. In hydrogen‐driven biotransformations, whole cells catalyzed asymmetric C=C bond reduction of unsaturated cyclic ketones with stereoselectivities up to >99% ee. The use of hydrogen as a substrate for growth and cofactor regeneration is particularly attractive as it represents a strategy for improving atom efficiency and reducing side product formation associated with the recycling of organic cofactors.
Original languageEnglish
Pages (from-to)2361-2365
Number of pages5
JournalChemSusChem
Volume12
Issue number11
Early online date19 Mar 2019
DOIs
Publication statusPublished - 19 Mar 2019

Keywords

  • Hydrogenation
  • Biotransformations
  • Biocatalysis
  • Assymetric synthesis
  • Reduction
  • biotransformation
  • asymmetric synthesis
  • reduction
  • hydrogenation
  • biocatalysis

ASJC Scopus subject areas

  • Energy(all)
  • Chemical Engineering(all)
  • Materials Science(all)
  • Environmental Chemistry

Fields of Expertise

  • Human- & Biotechnology

Fingerprint Dive into the research topics of 'Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator'. Together they form a unique fingerprint.

  • Cite this