Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

The coupling of recombinantly expressed oxidoreductases to endogenous hydrogenases for cofactor‐recycling permits the omission of organic cosubstrates as sacrificial electron donors in whole‐cell biotransformations. This increases atom efficiency and simplifies the reaction. We have expressed a recombinant ene‐reductase in the hydrogen‐oxidizing proteobacterium Cupriavidus necator H16. In hydrogen‐driven biotransformations, whole cells catalyzed asymmetric C=C bond reduction of unsaturated cyclic ketones with stereoselectivities up to >99% ee. The use of hydrogen as a substrate for growth and cofactor regeneration is particularly attractive as it represents a strategy for improving atom efficiency and reducing side product formation associated with the recycling of organic cofactors.
Originalspracheenglisch
Seiten (von - bis)2361-2365
Seitenumfang5
FachzeitschriftChemSusChem
Jahrgang12
Ausgabenummer11
Frühes Online-Datum19 Mär 2019
DOIs
PublikationsstatusVeröffentlicht - 19 Mär 2019

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biotransformation
Hydrogen
regeneration
hydrogen
Hydrogenase
Stereoselectivity
Atoms
ketone
Ketones
Recycling
Oxidoreductases
recycling
substrate
electron
Electrons
Substrates
Biotransformation
asoxime chloride
Proteobacteria
product

Schlagwörter

    ASJC Scopus subject areas

    • !!Energy(all)
    • !!Chemical Engineering(all)
    • !!Materials Science(all)
    • Umweltchemie

    Fields of Expertise

    • Human- & Biotechnology

    Dies zitieren

    Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator. / Assil Companioni, Leen; Schmidt, Sandy; Heidinger, Petra; Schwab, Helmut; Kourist, Robert.

    in: ChemSusChem, Jahrgang 12, Nr. 11, 19.03.2019, S. 2361-2365.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

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    abstract = "The coupling of recombinantly expressed oxidoreductases to endogenous hydrogenases for cofactor‐recycling permits the omission of organic cosubstrates as sacrificial electron donors in whole‐cell biotransformations. This increases atom efficiency and simplifies the reaction. We have expressed a recombinant ene‐reductase in the hydrogen‐oxidizing proteobacterium Cupriavidus necator H16. In hydrogen‐driven biotransformations, whole cells catalyzed asymmetric C=C bond reduction of unsaturated cyclic ketones with stereoselectivities up to >99{\%} ee. The use of hydrogen as a substrate for growth and cofactor regeneration is particularly attractive as it represents a strategy for improving atom efficiency and reducing side product formation associated with the recycling of organic cofactors.",
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    AU - Schmidt, Sandy

    AU - Heidinger, Petra

    AU - Schwab, Helmut

    AU - Kourist, Robert

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