Experimental investigation of laccase purification using aqueous two-phase extraction

Axel Prinz, Tim Zeiner*, Thomas Vössing, Ina Schüttmann, Holger Zorn, Andrzej Górak

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.

Original languageEnglish
Pages (from-to)349-354
Number of pages6
JournalChemical Engineering Transactions
Volume27
DOIs
Publication statusPublished - 2012

ASJC Scopus subject areas

  • Chemical Engineering(all)

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