Experimental investigation of laccase purification using aqueous two-phase extraction

Axel Prinz, Tim Zeiner, Thomas Vössing, Ina Schüttmann, Holger Zorn, Andrzej Górak

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung


In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.

Seiten (von - bis)349-354
FachzeitschriftChemical Engineering Transactions
PublikationsstatusVeröffentlicht - 2012


ASJC Scopus subject areas

  • !!Chemical Engineering(all)

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