Experimental investigation of laccase purification using aqueous two-phase extraction

Axel Prinz, Tim Zeiner, Thomas Vössing, Ina Schüttmann, Holger Zorn, Andrzej Górak

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.

Originalspracheenglisch
Seiten (von - bis)349-354
Seitenumfang6
FachzeitschriftChemical Engineering Transactions
Jahrgang27
DOIs
PublikationsstatusVeröffentlicht - 2012

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ASJC Scopus subject areas

  • !!Chemical Engineering(all)

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