Experimental investigation of laccase purification using aqueous two-phase extraction

Axel Prinz, Tim Zeiner, Thomas Vössing, Ina Schüttmann, Holger Zorn, Andrzej Górak

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.

Originalspracheenglisch
Seiten (von - bis)349-354
Seitenumfang6
FachzeitschriftChemical Engineering Transactions
Jahrgang27
DOIs
PublikationsstatusVeröffentlicht - 2012

Fingerprint

Laccase
Sodium chloride
Sodium Chloride
Purification
Enzyme activity
Polyethylene glycols
Fermentation
Potassium
Phosphates
Molecular weight
Sodium
Impurities

ASJC Scopus subject areas

  • !!Chemical Engineering(all)

Dies zitieren

Experimental investigation of laccase purification using aqueous two-phase extraction. / Prinz, Axel; Zeiner, Tim; Vössing, Thomas; Schüttmann, Ina; Zorn, Holger; Górak, Andrzej.

in: Chemical Engineering Transactions, Jahrgang 27, 2012, S. 349-354.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Prinz, Axel ; Zeiner, Tim ; Vössing, Thomas ; Schüttmann, Ina ; Zorn, Holger ; Górak, Andrzej. / Experimental investigation of laccase purification using aqueous two-phase extraction. in: Chemical Engineering Transactions. 2012 ; Jahrgang 27. S. 349-354.
@article{6f4573f673b042ed975c0b54afacb008,
title = "Experimental investigation of laccase purification using aqueous two-phase extraction",
abstract = "In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.",
author = "Axel Prinz and Tim Zeiner and Thomas V{\"o}ssing and Ina Sch{\"u}ttmann and Holger Zorn and Andrzej G{\'o}rak",
year = "2012",
doi = "10.3303/CET1227059",
language = "English",
volume = "27",
pages = "349--354",
journal = "Chemical Engineering Transactions",
issn = "2283-9216",
publisher = "AIDIC-Italian Association of Chemical Engineering",

}

TY - JOUR

T1 - Experimental investigation of laccase purification using aqueous two-phase extraction

AU - Prinz, Axel

AU - Zeiner, Tim

AU - Vössing, Thomas

AU - Schüttmann, Ina

AU - Zorn, Holger

AU - Górak, Andrzej

PY - 2012

Y1 - 2012

N2 - In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.

AB - In this paper the partitioning behavior of laccase from a culture supernatant of a Pleurotus sapidus fermentation in an aqueous two-phase system (ATPS) of polyethylene glycol with a molecular weight of 3000 g/mol (PEG 3000), potassium and sodium phosphate and sodium chloride is experimentally investigated. Results show that enzyme activity is retained during aqueous two-phase extraction. In addition the enzyme activity partitioning strongly depends on the sodium chloride concentration. The partitioning of impurities is not influenced with varying amounts of sodium chloride.

UR - http://www.scopus.com/inward/record.url?scp=84864405400&partnerID=8YFLogxK

U2 - 10.3303/CET1227059

DO - 10.3303/CET1227059

M3 - Article

VL - 27

SP - 349

EP - 354

JO - Chemical Engineering Transactions

JF - Chemical Engineering Transactions

SN - 2283-9216

ER -