Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

Yusuke Miyauchi; Robert Kourist; Daisuke Uemura; Kenji Miyamoto

doi:10.1039/c1cc11953b

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

Yusuke Miyauchi, Robert Kourist, Daisuke Uemura, Kenji Miyamoto

Institute of Molecular Biotechnology (6550)

Research output: Contribution to journal › Article › peer-review

Abstract

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).

Original language	English
Pages (from-to)	7503-7505
Number of pages	3
Journal	Chemical Communications
Volume	47
DOIs	https://doi.org/10.1039/c1cc11953b
Publication status	Published - 2011

Keywords

Biocatalysis Bordetella bronchiseptica Bordetella bronchiseptica: enzymology Carboxy-Lyases Carboxy-Lyases: chemistry Carboxy-Lyases: genetics Carboxy-Lyases: metabolism Catalytic Domain Directed Molecular Evolution Directed Molecular Evolution: methods Models, Molecular Molecular Mutation Substrate Specificity Sulfur Sulfur: metabolism

Access to Document

10.1039/c1cc11953b

Cite this

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title = "Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.",

abstract = "Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).",

keywords = "Biocatalysis Bordetella bronchiseptica Bordetella bronchiseptica: enzymology Carboxy-Lyases Carboxy-Lyases: chemistry Carboxy-Lyases: genetics Carboxy-Lyases: metabolism Catalytic Domain Directed Molecular Evolution Directed Molecular Evolution: methods Models, Molecular Molecular Mutation Substrate Specificity Sulfur Sulfur: metabolism",

author = "Yusuke Miyauchi and Robert Kourist and Daisuke Uemura and Kenji Miyamoto",

year = "2011",

doi = "10.1039/c1cc11953b",

language = "English",

volume = "47",

pages = "7503--7505",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

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T1 - Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

AU - Miyauchi, Yusuke

AU - Kourist, Robert

AU - Uemura, Daisuke

AU - Miyamoto, Kenji

PY - 2011

Y1 - 2011

N2 - Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).

AB - Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).

KW - Biocatalysis Bordetella bronchiseptica Bordetella bronchiseptica: enzymology Carboxy-Lyases Carboxy-Lyases: chemistry Carboxy-Lyases: genetics Carboxy-Lyases: metabolism Catalytic Domain Directed Molecular Evolution Directed Molecular Evolution: methods M

U2 - 10.1039/c1cc11953b

DO - 10.1039/c1cc11953b

M3 - Article

SN - 1359-7345

VL - 47

SP - 7503

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JO - Chemical Communications

JF - Chemical Communications

ER -