Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

Yusuke Miyauchi, Robert Kourist, Daisuke Uemura, Kenji Miyamoto

Publikation: Beitrag in einer FachzeitschriftArtikel

Abstract

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).
Originalspracheenglisch
Seiten (von - bis)7503-7505
Seitenumfang3
FachzeitschriftChemical Communications
Jahrgang47
DOIs
PublikationsstatusVeröffentlicht - 2011

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