Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

Yusuke Miyauchi, Robert Kourist, Daisuke Uemura, Kenji Miyamoto

Research output: Contribution to journalArticle

Abstract

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).
LanguageEnglish
Pages7503-7505
Number of pages3
JournalChemical communications
Volume47
DOIs
StatusPublished - 2011

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Naproxen
malonate decarboxylase

Keywords

  • Biocatalysis Bordetella bronchiseptica Bordetella bronchiseptica: enzymology Carboxy-Lyases Carboxy-Lyases: chemistry Carboxy-Lyases: genetics Carboxy-Lyases: metabolism Catalytic Domain Directed Molecular Evolution Directed Molecular Evolution: methods Models, Molecular Molecular Mutation Substrate Specificity Sulfur Sulfur: metabolism

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Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution. / Miyauchi, Yusuke; Kourist, Robert; Uemura, Daisuke; Miyamoto, Kenji.

In: Chemical communications, Vol. 47, 2011, p. 7503-7505.

Research output: Contribution to journalArticle

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AU - Miyamoto,Kenji

PY - 2011

Y1 - 2011

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