Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

Yusuke Miyauchi, Robert Kourist, Daisuke Uemura, Kenji Miyamoto

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).
Original languageEnglish
Pages (from-to)7503-7505
Number of pages3
JournalChemical Communications
Volume47
DOIs
Publication statusPublished - 2011

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Keywords

  • Biocatalysis Bordetella bronchiseptica Bordetella bronchiseptica: enzymology Carboxy-Lyases Carboxy-Lyases: chemistry Carboxy-Lyases: genetics Carboxy-Lyases: metabolism Catalytic Domain Directed Molecular Evolution Directed Molecular Evolution: methods Models, Molecular Molecular Mutation Substrate Specificity Sulfur Sulfur: metabolism

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