Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).
- Biocatalysis Bordetella bronchiseptica Bordetella bronchiseptica: enzymology Carboxy-Lyases Carboxy-Lyases: chemistry Carboxy-Lyases: genetics Carboxy-Lyases: metabolism Catalytic Domain Directed Molecular Evolution Directed Molecular Evolution: methods Models, Molecular Molecular Mutation Substrate Specificity Sulfur Sulfur: metabolism
Miyauchi, Y., Kourist, R., Uemura, D., & Miyamoto, K. (2011). Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution. Chemical Communications, 47, 7503-7505. https://doi.org/10.1039/c1cc11953b