Design of simultaneous antimicrobial and anticoagulant surfaces based on nanoparticles and polysaccharides†

Doris Breitwieser, Stefan Spirk, Hubert Fasl, Heike M.A. Ehmann, Angela Chemelli, Victoria Reichel, Christian Gspan, Karin Stana-Kleinschek, Volker Ribitsch

Research output: Contribution to journalArticle

Abstract

The rational design of silver nanoparticles encapsulated in an anticoagulant, hemocompatible polysaccharide, 6-O-chitosan sulfate, is presented. Three different approaches are described for the immobilization of these core shell particles on cellulosic surfaces. The mass of the immobilized particles is quantified using a quartz crystal microbalance with dissipation (QCM-D). The antimicrobial activity of the surfaces towards E. coli MG 1655 [R1-16] is investigated by live/dead assays using fluorescence staining. All surfaces treated with the designed nanoparticles exhibit excellent antimicrobial activity towards E. coli MG 1655 [R1-16]. Anticoagulant properties of blood plasma on the nanoparticle treated surfaces have been determined using QCM-D. In comparison with the unmodified substrates, the total coagulation time as well as the thrombin formation time and fibrin clotting time of surfaces modified with nanoparticles are significantly increased.
Original languageEnglish
Pages (from-to)2022-2030
Number of pages9
JournalJournal of materials chemistry / B
Volume1
Issue number15
DOIs
Publication statusPublished - 2013

Fields of Expertise

  • Advanced Materials Science

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)
  • Application
  • Experimental

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