Design of simultaneous antimicrobial and anticoagulant surfaces based on nanoparticles and polysaccharides†

Doris Breitwieser, Stefan Spirk, Hubert Fasl, Heike M.A. Ehmann, Angela Chemelli, Victoria Reichel, Christian Gspan, Karin Stana-Kleinschek, Volker Ribitsch

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

The rational design of silver nanoparticles encapsulated in an anticoagulant, hemocompatible polysaccharide, 6-O-chitosan sulfate, is presented. Three different approaches are described for the immobilization of these core shell particles on cellulosic surfaces. The mass of the immobilized particles is quantified using a quartz crystal microbalance with dissipation (QCM-D). The antimicrobial activity of the surfaces towards E. coli MG 1655 [R1-16] is investigated by live/dead assays using fluorescence staining. All surfaces treated with the designed nanoparticles exhibit excellent antimicrobial activity towards E. coli MG 1655 [R1-16]. Anticoagulant properties of blood plasma on the nanoparticle treated surfaces have been determined using QCM-D. In comparison with the unmodified substrates, the total coagulation time as well as the thrombin formation time and fibrin clotting time of surfaces modified with nanoparticles are significantly increased.
Originalspracheenglisch
Seiten (von - bis)2022-2030
Seitenumfang9
FachzeitschriftJournal of materials chemistry / B
Jahrgang1
Ausgabenummer15
DOIs
PublikationsstatusVeröffentlicht - 2013

Fields of Expertise

  • Advanced Materials Science

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)
  • Application
  • Experimental

Dies zitieren

Design of simultaneous antimicrobial and anticoagulant surfaces based on nanoparticles and polysaccharides†. / Breitwieser, Doris; Spirk, Stefan; Fasl, Hubert; Ehmann, Heike M.A.; Chemelli, Angela; Reichel, Victoria; Gspan, Christian; Stana-Kleinschek, Karin; Ribitsch, Volker.

in: Journal of materials chemistry / B, Jahrgang 1, Nr. 15, 2013, S. 2022-2030.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Breitwieser, D, Spirk, S, Fasl, H, Ehmann, HMA, Chemelli, A, Reichel, V, Gspan, C, Stana-Kleinschek, K & Ribitsch, V 2013, 'Design of simultaneous antimicrobial and anticoagulant surfaces based on nanoparticles and polysaccharides†' Journal of materials chemistry / B, Jg. 1, Nr. 15, S. 2022-2030. https://doi.org/10.1039/c3tb00272a, https://doi.org/10.1039/C3TB00272A
Breitwieser, Doris ; Spirk, Stefan ; Fasl, Hubert ; Ehmann, Heike M.A. ; Chemelli, Angela ; Reichel, Victoria ; Gspan, Christian ; Stana-Kleinschek, Karin ; Ribitsch, Volker. / Design of simultaneous antimicrobial and anticoagulant surfaces based on nanoparticles and polysaccharides†. in: Journal of materials chemistry / B. 2013 ; Jahrgang 1, Nr. 15. S. 2022-2030.
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abstract = "The rational design of silver nanoparticles encapsulated in an anticoagulant, hemocompatible polysaccharide, 6-O-chitosan sulfate, is presented. Three different approaches are described for the immobilization of these core shell particles on cellulosic surfaces. The mass of the immobilized particles is quantified using a quartz crystal microbalance with dissipation (QCM-D). The antimicrobial activity of the surfaces towards E. coli MG 1655 [R1-16] is investigated by live/dead assays using fluorescence staining. All surfaces treated with the designed nanoparticles exhibit excellent antimicrobial activity towards E. coli MG 1655 [R1-16]. Anticoagulant properties of blood plasma on the nanoparticle treated surfaces have been determined using QCM-D. In comparison with the unmodified substrates, the total coagulation time as well as the thrombin formation time and fibrin clotting time of surfaces modified with nanoparticles are significantly increased.",
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T1 - Design of simultaneous antimicrobial and anticoagulant surfaces based on nanoparticles and polysaccharides†

AU - Breitwieser, Doris

AU - Spirk, Stefan

AU - Fasl, Hubert

AU - Ehmann, Heike M.A.

AU - Chemelli, Angela

AU - Reichel, Victoria

AU - Gspan, Christian

AU - Stana-Kleinschek, Karin

AU - Ribitsch, Volker

PY - 2013

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N2 - The rational design of silver nanoparticles encapsulated in an anticoagulant, hemocompatible polysaccharide, 6-O-chitosan sulfate, is presented. Three different approaches are described for the immobilization of these core shell particles on cellulosic surfaces. The mass of the immobilized particles is quantified using a quartz crystal microbalance with dissipation (QCM-D). The antimicrobial activity of the surfaces towards E. coli MG 1655 [R1-16] is investigated by live/dead assays using fluorescence staining. All surfaces treated with the designed nanoparticles exhibit excellent antimicrobial activity towards E. coli MG 1655 [R1-16]. Anticoagulant properties of blood plasma on the nanoparticle treated surfaces have been determined using QCM-D. In comparison with the unmodified substrates, the total coagulation time as well as the thrombin formation time and fibrin clotting time of surfaces modified with nanoparticles are significantly increased.

AB - The rational design of silver nanoparticles encapsulated in an anticoagulant, hemocompatible polysaccharide, 6-O-chitosan sulfate, is presented. Three different approaches are described for the immobilization of these core shell particles on cellulosic surfaces. The mass of the immobilized particles is quantified using a quartz crystal microbalance with dissipation (QCM-D). The antimicrobial activity of the surfaces towards E. coli MG 1655 [R1-16] is investigated by live/dead assays using fluorescence staining. All surfaces treated with the designed nanoparticles exhibit excellent antimicrobial activity towards E. coli MG 1655 [R1-16]. Anticoagulant properties of blood plasma on the nanoparticle treated surfaces have been determined using QCM-D. In comparison with the unmodified substrates, the total coagulation time as well as the thrombin formation time and fibrin clotting time of surfaces modified with nanoparticles are significantly increased.

U2 - 10.1039/c3tb00272a

DO - 10.1039/c3tb00272a

M3 - Article

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JO - Journal of materials chemistry / B

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