Abstract
Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF 4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.
Originalsprache | englisch |
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Seiten (von - bis) | 12441-12463 |
Seitenumfang | 23 |
Fachzeitschrift | Molecules |
Jahrgang | 18 |
Ausgabenummer | 10 |
DOIs | |
Publikationsstatus | Veröffentlicht - 1 Okt. 2013 |
Extern publiziert | Ja |
ASJC Scopus subject areas
- Analytische Chemie
- Chemie (sonstige)
- Molekularmedizin
- Pharmazeutische Wissenschaften
- Wirkstoffforschung
- Physikalische und Theoretische Chemie
- Organische Chemie