LOVTRAP: an optogenetic system for photoinduced protein dissociation

Hui Wang, Marco Vilela, Andreas Winkler, Miroslaw Tarnawski, Ilme Schlichting, Hayretin Yumerefendi, Brian Kuhlman, Rihe Liu, Gaudenz Danuser, Klaus M Hahn

Publikation: Beitrag in einer FachzeitschriftArtikel

Abstract

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.
Originalspracheenglisch
Seiten (von - bis)755-758
FachzeitschriftNature methods
Jahrgang13
DOIs
PublikationsstatusVeröffentlicht - 2016

ASJC Scopus subject areas

  • !!Biochemistry, Genetics and Molecular Biology (miscellaneous)

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