LOVTRAP: an optogenetic system for photoinduced protein dissociation

Hui Wang, Marco Vilela, Andreas Winkler, Miroslaw Tarnawski, Ilme Schlichting, Hayretin Yumerefendi, Brian Kuhlman, Rihe Liu, Gaudenz Danuser, Klaus M Hahn

Research output: Contribution to journalArticleResearchpeer-review

Abstract

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.
Original languageEnglish
Pages (from-to)755-758
JournalNature methods
Volume13
DOIs
Publication statusPublished - 2016

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Keywords

  • optogenetics
  • cell signalling
  • cell migration

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Wang, H., Vilela, M., Winkler, A., Tarnawski, M., Schlichting, I., Yumerefendi, H., ... Hahn, K. M. (2016). LOVTRAP: an optogenetic system for photoinduced protein dissociation. Nature methods, 13, 755-758. https://doi.org/10.1038/nmeth.3926