A synthetic biology approach for the transformation of L-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

Geoffrey Gourinchas; Eduardo Busto; Manuela Killinger; Nina Richter; Birgit Wiltschi; Wolfgang Kroutil

doi:10.1039/c4cc08286a

A synthetic biology approach for the transformation of L-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

Geoffrey Gourinchas, Eduardo Busto, Manuela Killinger, Nina Richter, Birgit Wiltschi^*, Wolfgang Kroutil^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Technische Universität Graz (90000)

Publikation: Beitrag in einer Fachzeitschrift › Artikel › Begutachtung

Abstract

Combinatorial assembly and variation of promoters on a single expression plasmid allowed the balance of the catalytic steps of a three enzyme (l-AAD, HIC, FDH) cascade in E. coli. The designer cell catalyst quantitatively transformed l-amino acids to the corresponding optically pure (R)- and (S)-α-hydroxy acids at up to 200 mM substrate concentration.

Originalsprache	englisch
Seiten (von - bis)	2828-2831
Seitenumfang	4
Fachzeitschrift	Chemical Communications
Jahrgang	51
Ausgabenummer	14
DOIs	https://doi.org/10.1039/c4cc08286a
Publikationsstatus	Veröffentlicht - 9 Jan. 2015

ASJC Scopus subject areas

Katalyse
Elektronische, optische und magnetische Materialien
Keramische und Verbundwerkstoffe
Allgemeine Chemie
Oberflächen, Beschichtungen und Folien
Metalle und Legierungen
Werkstoffchemie

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10.1039/c4cc08286a

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author = "Geoffrey Gourinchas and Eduardo Busto and Manuela Killinger and Nina Richter and Birgit Wiltschi and Wolfgang Kroutil",

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AU - Busto, Eduardo

AU - Killinger, Manuela

AU - Richter, Nina

AU - Wiltschi, Birgit

AU - Kroutil, Wolfgang

PY - 2015/1/9

Y1 - 2015/1/9

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AB - Combinatorial assembly and variation of promoters on a single expression plasmid allowed the balance of the catalytic steps of a three enzyme (l-AAD, HIC, FDH) cascade in E. coli. The designer cell catalyst quantitatively transformed l-amino acids to the corresponding optically pure (R)- and (S)-α-hydroxy acids at up to 200 mM substrate concentration.

UR - http://www.scopus.com/inward/record.url?scp=84922351028&partnerID=8YFLogxK

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A synthetic biology approach for the transformation of L-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

Abstract

ASJC Scopus subject areas

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