A synthetic biology approach for the transformation of L-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids

Geoffrey Gourinchas, Eduardo Busto, Manuela Killinger, Nina Richter, Birgit Wiltschi*, Wolfgang Kroutil*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Combinatorial assembly and variation of promoters on a single expression plasmid allowed the balance of the catalytic steps of a three enzyme (l-AAD, HIC, FDH) cascade in E. coli. The designer cell catalyst quantitatively transformed l-amino acids to the corresponding optically pure (R)- and (S)-α-hydroxy acids at up to 200 mM substrate concentration.

Original languageEnglish
Pages (from-to)2828-2831
Number of pages4
JournalChemical Communications
Volume51
Issue number14
DOIs
Publication statusPublished - 9 Jan 2015

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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