Stability and disassembly properties of human naïve Hsp60 and bacterial GroEL chaperonins

Caterina Ricci, Maria Grazia Ortore, Silvia Vilasi, Rita Carrotta, Maria Rosalia Mangione, Donatella Bulone, Fabio Librizzi, Francesco Spinozzi, Giosalba Burgio, Heinz Amenitsch, Pier Luigi San Biagio

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Human Hsp60 chaperonin and its bacterial homolog GroEL, in association with the corresponding co-chaperonins Hsp10 and GroES, constitute important chaperone systems promoting the proper folding of several mitochondrial proteins. Hsp60 is also currently described as a ubiquitous molecule with multiple roles both in health conditions and in several diseases. Naïve Hsp60 bearing the mitochondrial import signal has been recently demonstrated to present different oligomeric organizations with respect to GroEL, suggesting new possible physiological functions. Here we present a combined investigation with circular dichroism and small-angle X-ray scattering of structure, self-organization, and stability of naïve Hsp60 in solution in comparison with bacterial GroEL. Experiments have been performed in different concentrations of guanidine hydrochloride, monitoring the dissociation of tetradecamers into heptamers and monomers, until unfolding. GroEL is proved to be more stable with respect to Hsp60, and the unfolding free energy as well as its dependence on denaturant concentration is obtained.

Original languageEnglish
Pages (from-to)68-75
Number of pages8
JournalBiophysical Chemistry
Volume208
DOIs
Publication statusPublished - 1 Jan 2016

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Keywords

  • CD
  • Denaturation
  • GroEL
  • Guanidinium chloride
  • Hsp60
  • SAXS

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Organic Chemistry

Cite this

Ricci, C., Ortore, M. G., Vilasi, S., Carrotta, R., Mangione, M. R., Bulone, D., ... San Biagio, P. L. (2016). Stability and disassembly properties of human naïve Hsp60 and bacterial GroEL chaperonins. Biophysical Chemistry, 208, 68-75. https://doi.org/10.1016/j.bpc.2015.07.006