TY - JOUR
T1 - Nitrile hydrolysis activity of Rhodococcus erythropolis NCIMB 11540 whole cells.
AU - Vink, Mandy K.S.
AU - Wijtmans, R
AU - Reisinger, C
AU - Van den Berg, Rutger J.F.
AU - Schortinghuis, Christien A.
AU - Schwab, Helmut
AU - Schoemaker, Hans E.
AU - Rutjes, Floris P.J.T.
PY - 2006
Y1 - 2006
N2 - The nitrile hydrolyzing properties of the bacterium strain Rhodococcus erythropolis NCIMB 11540 have been investigated. Using whole cells of the microorganism, a wide variety of aromatic and aliphatic cyanide-containing substrates was successfully hydrolyzed to the corresponding amide or acid. In the case of dicyanides, selective monohydrolysis took place, which was further explored in the desymmetrization of malononitriles resulting in the corresponding cyano amides in enantiomeric excesses of up to 98%.
AB - The nitrile hydrolyzing properties of the bacterium strain Rhodococcus erythropolis NCIMB 11540 have been investigated. Using whole cells of the microorganism, a wide variety of aromatic and aliphatic cyanide-containing substrates was successfully hydrolyzed to the corresponding amide or acid. In the case of dicyanides, selective monohydrolysis took place, which was further explored in the desymmetrization of malononitriles resulting in the corresponding cyano amides in enantiomeric excesses of up to 98%.
U2 - 10.1002/biot.200600028
DO - 10.1002/biot.200600028
M3 - Article
SN - 0168-1656
VL - 1
SP - 569
EP - 573
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 5
ER -