Lipidomics by ultrahigh performance liquid chromatography-high resolution mass spectrometry and its application to complex biological samples

Alexander Triebl, M. Trötzmüller, Jürgen Hartler, Tatjana Stojakovic, Harald C. Köfeler

Research output: Contribution to journalArticleResearchpeer-review

Abstract

An improved approach for selective and sensitive identification and quantitation of lipid molecular species using reversed phase chromatography coupled to high resolution mass spectrometry was developed. The method is applicable to a wide variety of biological matrices using a simple liquid-liquid extraction procedure. Together, this approach combines multiple selectivity criteria: Reversed phase chromatography separates lipids according to their acyl chain length and degree of unsaturation and is capable of resolving positional isomers of lysophospholipids, as well as structural isomers of diacyl phospholipids and glycerolipids. Orbitrap mass spectrometry delivers the elemental composition of both positive and negative ions with high mass accuracy. Finally, automatically generated tandem mass spectra provide structural insight into numerous glycerolipids, phospholipids, and sphingolipids within a single run. Calibration showed linearity ranges of more than four orders of magnitude, good values for accuracy and precision at biologically relevant concentration levels, and limits of quantitation of a few femtomoles on column. Hundreds of lipid molecular species were detected and quantified in three different biological matrices, which cover well the wide variety and complexity of various model organisms in lipidomic research. Together with a software package, this method is a prime choice for global lipidomic analysis of even the most complex biological samples.

Original languageEnglish
Pages (from-to)72-80
Number of pages9
JournalJournal of chromatography / B
Volume1053
DOIs
Publication statusPublished - 15 May 2017

Fingerprint

High performance liquid chromatography
Mass spectrometry
Mass Spectrometry
High Pressure Liquid Chromatography
Reverse-Phase Chromatography
Chromatography
Lipids
Isomers
Phospholipids
Lysophospholipids
Liquid-Liquid Extraction
Sphingolipids
Liquids
Chain length
Software packages
Calibration
Negative ions
Software
Positive ions
Ions

Keywords

  • Animals
  • Caenorhabditis elegans
  • Cell Line, Tumor
  • Chromatography, High Pressure Liquid
  • Chromatography, Reverse-Phase
  • Humans
  • Isomerism
  • Lipids
  • Liquid-Liquid Extraction
  • Mass Spectrometry
  • Metabolomics
  • Mice
  • Journal Article

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Experimental

Cite this

Lipidomics by ultrahigh performance liquid chromatography-high resolution mass spectrometry and its application to complex biological samples. / Triebl, Alexander; Trötzmüller, M.; Hartler, Jürgen; Stojakovic, Tatjana; Köfeler, Harald C.

In: Journal of chromatography / B, Vol. 1053, 15.05.2017, p. 72-80.

Research output: Contribution to journalArticleResearchpeer-review

@article{b8cf518582fc4e1a8a66f36fdde101cd,
title = "Lipidomics by ultrahigh performance liquid chromatography-high resolution mass spectrometry and its application to complex biological samples",
abstract = "An improved approach for selective and sensitive identification and quantitation of lipid molecular species using reversed phase chromatography coupled to high resolution mass spectrometry was developed. The method is applicable to a wide variety of biological matrices using a simple liquid-liquid extraction procedure. Together, this approach combines multiple selectivity criteria: Reversed phase chromatography separates lipids according to their acyl chain length and degree of unsaturation and is capable of resolving positional isomers of lysophospholipids, as well as structural isomers of diacyl phospholipids and glycerolipids. Orbitrap mass spectrometry delivers the elemental composition of both positive and negative ions with high mass accuracy. Finally, automatically generated tandem mass spectra provide structural insight into numerous glycerolipids, phospholipids, and sphingolipids within a single run. Calibration showed linearity ranges of more than four orders of magnitude, good values for accuracy and precision at biologically relevant concentration levels, and limits of quantitation of a few femtomoles on column. Hundreds of lipid molecular species were detected and quantified in three different biological matrices, which cover well the wide variety and complexity of various model organisms in lipidomic research. Together with a software package, this method is a prime choice for global lipidomic analysis of even the most complex biological samples.",
keywords = "Animals, Caenorhabditis elegans, Cell Line, Tumor, Chromatography, High Pressure Liquid, Chromatography, Reverse-Phase, Humans, Isomerism, Lipids, Liquid-Liquid Extraction, Mass Spectrometry, Metabolomics, Mice, Journal Article",
author = "Alexander Triebl and M. Tr{\"o}tzm{\"u}ller and J{\"u}rgen Hartler and Tatjana Stojakovic and K{\"o}feler, {Harald C.}",
note = "Copyright {\circledC} 2017 Elsevier B.V. All rights reserved.",
year = "2017",
month = "5",
day = "15",
doi = "10.1016/j.jchromb.2017.03.027",
language = "English",
volume = "1053",
pages = "72--80",
journal = "Journal of chromatography / B",
issn = "1570-0232",
publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Lipidomics by ultrahigh performance liquid chromatography-high resolution mass spectrometry and its application to complex biological samples

AU - Triebl, Alexander

AU - Trötzmüller, M.

AU - Hartler, Jürgen

AU - Stojakovic, Tatjana

AU - Köfeler, Harald C.

N1 - Copyright © 2017 Elsevier B.V. All rights reserved.

PY - 2017/5/15

Y1 - 2017/5/15

N2 - An improved approach for selective and sensitive identification and quantitation of lipid molecular species using reversed phase chromatography coupled to high resolution mass spectrometry was developed. The method is applicable to a wide variety of biological matrices using a simple liquid-liquid extraction procedure. Together, this approach combines multiple selectivity criteria: Reversed phase chromatography separates lipids according to their acyl chain length and degree of unsaturation and is capable of resolving positional isomers of lysophospholipids, as well as structural isomers of diacyl phospholipids and glycerolipids. Orbitrap mass spectrometry delivers the elemental composition of both positive and negative ions with high mass accuracy. Finally, automatically generated tandem mass spectra provide structural insight into numerous glycerolipids, phospholipids, and sphingolipids within a single run. Calibration showed linearity ranges of more than four orders of magnitude, good values for accuracy and precision at biologically relevant concentration levels, and limits of quantitation of a few femtomoles on column. Hundreds of lipid molecular species were detected and quantified in three different biological matrices, which cover well the wide variety and complexity of various model organisms in lipidomic research. Together with a software package, this method is a prime choice for global lipidomic analysis of even the most complex biological samples.

AB - An improved approach for selective and sensitive identification and quantitation of lipid molecular species using reversed phase chromatography coupled to high resolution mass spectrometry was developed. The method is applicable to a wide variety of biological matrices using a simple liquid-liquid extraction procedure. Together, this approach combines multiple selectivity criteria: Reversed phase chromatography separates lipids according to their acyl chain length and degree of unsaturation and is capable of resolving positional isomers of lysophospholipids, as well as structural isomers of diacyl phospholipids and glycerolipids. Orbitrap mass spectrometry delivers the elemental composition of both positive and negative ions with high mass accuracy. Finally, automatically generated tandem mass spectra provide structural insight into numerous glycerolipids, phospholipids, and sphingolipids within a single run. Calibration showed linearity ranges of more than four orders of magnitude, good values for accuracy and precision at biologically relevant concentration levels, and limits of quantitation of a few femtomoles on column. Hundreds of lipid molecular species were detected and quantified in three different biological matrices, which cover well the wide variety and complexity of various model organisms in lipidomic research. Together with a software package, this method is a prime choice for global lipidomic analysis of even the most complex biological samples.

KW - Animals

KW - Caenorhabditis elegans

KW - Cell Line, Tumor

KW - Chromatography, High Pressure Liquid

KW - Chromatography, Reverse-Phase

KW - Humans

KW - Isomerism

KW - Lipids

KW - Liquid-Liquid Extraction

KW - Mass Spectrometry

KW - Metabolomics

KW - Mice

KW - Journal Article

U2 - 10.1016/j.jchromb.2017.03.027

DO - 10.1016/j.jchromb.2017.03.027

M3 - Article

VL - 1053

SP - 72

EP - 80

JO - Journal of chromatography / B

JF - Journal of chromatography / B

SN - 1570-0232

ER -