High-throughput screening in protein engineering: Recent advances and future perspectives

Magdalena Wójcik, Aline Telzerow, Wim J. Quax, Ykelien L. Boersma

Research output: Contribution to journalReview articleResearchpeer-review

Abstract

Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms.

Original languageEnglish
Pages (from-to)24918-24945
Number of pages28
JournalInternational Journal of Molecular Sciences
Volume16
Issue number10
DOIs
Publication statusPublished - 20 Oct 2015
Externally publishedYes

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Keywords

  • FACS-based screening platforms
  • High-throughput screening
  • In vitro compartmentalization
  • Microfluidics-based screening platforms
  • Protein engineering

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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