Fast pesticide detection inside microfluidic device with integrated optical pH, oxygen sensors and algal fluorescence

Islam Bogachan Tahirbegi, Josef Ehgartner, Philipp Sulzer, Silvia Zieger, Alice Kasjanow, Mirco Paradiso, Martin Strobl, Dominique Bouwes, Torsten Mayr

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The necessities of developing fast, portable, cheap and easy to handle pesticide detection platforms are getting attention of scientific and industrial communities. Although there are some approaches to develop microchip based pesticide detection platforms, there is no compact microfluidic device for the complementary, fast, cheap, reusable and reliable analysis of different pesticides. In this work, a microfluidic device is developed for in-situ analysis of pesticide concentration detected via metabolism/photosynthesis of Chlamydomonas reinhardtii algal cells (algae) in tap water. Algae are grown in glass based microfluidic chip, which contains integrated optical pH and oxygen sensors in a portable system for on-site detection. In addition, intrinsic algal fluorescence is detected to analyze the pesticide concentration in parallel to pH and oxygen sensors with integrated fluorescence detectors. The response of the algae under the effect of different concentrations of pesticides is evaluated and complementary inhibition effects depending on the pesticide concentration are demonstrated. The three different sensors allow the determination of various pesticide concentrations in the nanomolar concentration range. The miniaturized system provides the fast quantification of pesticides in less than 10min and enables the study of toxic effects of different pesticides on Chlamydomonas reinhardtii green algae. Consequently, the microfluidic device described here provides fast and complementary detection of different pesticides with algae in a novel glass based microfluidic device with integrated optical pH, oxygen sensors and algal fluorescence.

LanguageEnglish
Pages188-195
JournalBiosensors & Bioelectronics
Volume88
DOIs
StatusPublished - 5 Aug 2017

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Lab-On-A-Chip Devices
Oxygen sensors
Pesticides
Microfluidics
Fluorescence
Oxygen
Algae
pH sensors
Chlamydomonas reinhardtii
Glass
Chlorophyta

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Fast pesticide detection inside microfluidic device with integrated optical pH, oxygen sensors and algal fluorescence. / Tahirbegi, Islam Bogachan; Ehgartner, Josef; Sulzer, Philipp; Zieger, Silvia; Kasjanow, Alice; Paradiso, Mirco; Strobl, Martin; Bouwes, Dominique; Mayr, Torsten.

In: Biosensors & Bioelectronics, Vol. 88, 05.08.2017, p. 188-195.

Research output: Contribution to journalArticleResearchpeer-review

Tahirbegi, Islam Bogachan ; Ehgartner, Josef ; Sulzer, Philipp ; Zieger, Silvia ; Kasjanow, Alice ; Paradiso, Mirco ; Strobl, Martin ; Bouwes, Dominique ; Mayr, Torsten. / Fast pesticide detection inside microfluidic device with integrated optical pH, oxygen sensors and algal fluorescence. In: Biosensors & Bioelectronics. 2017 ; Vol. 88. pp. 188-195.
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