Dimerization of the major birch pollen allergen bet v 1 is important for its in vivo IgE-cross-linking potential in mice

Isabella Schöll, Narayana Kalkura, Yuliya Shedziankova, Alexander Bergmann, Petra Verdino, Regina Knittelfelder, Tamara Kopp, Brigitte Hantusch, Christian Betzel, Rarsten Dierks, Otto Scheiner, George Boltz-Nitulescu, Walter Keller, Erika Jensen-Jarolim*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

In type 1 allergy, the cross-linking of membrane IgE on B lymphocytes and of cytophilic IgE on effector cells by their respective allergens are key events. For cross-linking two IgE molecules, allergens need at least two epitopes. On large molecules, these could be different epitopes in a multivalent, or identical epitopes in a symmetrical, fashion. However, the availability of epitopes may be limited on small allergens such as Bet v 1, the major birch pollen allergen. The present work analyzes whether dimerization is required for the cross-linking capacity of this allergen. In immunoblots, murine monoclonal and polyclonal human Bet v 1-specific Abs detected, besides a Bet v 1 monomer of 17 kDa, a dimer of 34 kDa. In dynamic light scattering, Bet v 1 appeared as dimers and even multimers, but a single condition could be defined where it behaved exclusively nionomerically. Small-angle x-ray scattering of the monomeric and dimeric samples resulted in diagrams agreeing with the calculated models. Circular dichroism measurements indicated that the structure of Bet v 1 was preserved under nionomeric conditions. Skin tests in Bet v 1-allergic mice were positive with Bet v 1 dimer, but remained negative using the monomer. Furthermore, in contrast to dimeric Bet v 1, the monomer was less capable of activating murine memory B cells for IgE production in vivo. Our data indicate that the presentation of two identical epitopes by dimerized allergens is a precondition for cross-linking of IgE on mast cells and B lymphocytes.

Original languageEnglish
Pages (from-to)6645-6650
Number of pages6
JournalThe Journal of Immunology
Volume175
Issue number10
DOIs
Publication statusPublished - 15 Nov 2005

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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