Current advances in engineering tools for Pichia pastoris

Jasmin E. Fischer, Anton Glieder

Research output: Contribution to journalReview articleResearchpeer-review

Abstract

In the past two to five years innovative DNA tools and inventive methodologies accelerated the speed of engineering of Komagataella phaffii (Pichia pastoris) for the efficient expression of intracellular and secreted proteins. Going beyond the standard approaches employing single heterologous genes or simultaneous expression of several different genes under the control of identical promoter and terminator sequences, balanced and consecutive co-expression of multiple genes (a, b) combined with simple host genome editing (c) now opens new opportunities for manufacturing of recombinant proteins or chemicals made by whole cell biocatalysis or synthetic biology.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalCurrent opinion in biotechnology
Volume59
DOIs
Publication statusPublished - 1 Oct 2019

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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