Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond

Fauziah F Rochman, Andriy Sheremet, Ivica Tamas, Alireza Saidi-Mehrabad, Joong-Jae Kim, Xiaoli Dong, Christoph W Sensen, Lisa M Gieg, Peter F Dunfield

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Oil sands process-affected water (OSPW), produced by surface-mining of oil sands in Canada, is alkaline and contains high concentrations of salts, metals, naphthenic acids, and polycyclic aromatic compounds (PAHs). Residual hydrocarbon biodegradation occurs naturally, but little is known about the hydrocarbon-degrading microbial communities present in OSPW. In this study, aerobic oxidation of benzene and naphthalene in the surface layer of an oil sands tailings pond were measured. The potential oxidation rates were 4.3 μmol L(-1) OSPW d(-1) for benzene and 21.4 μmol L(-1) OSPW d(-1) for naphthalene. To identify benzene and naphthalene-degrading microbial communities, metagenomics was combined with stable isotope probing (SIP), high-throughput sequencing of 16S rRNA gene amplicons, and isolation of microbial strains. SIP using (13)C-benzene and (13)C-naphthalene detected strains of the genera Methyloversatilis and Zavarzinia as the main benzene degraders, while strains belonging to the family Chromatiaceae and the genus Thauera were the main naphthalene degraders. Metagenomic analysis revealed a diversity of genes encoding oxygenases active against aromatic compounds. Although these genes apparently belonged to many phylogenetically diverse taxa, only a few of these taxa were predominant in the SIP experiments. This suggested that many members of the community are adapted to consuming other aromatic compounds, or are active only under specific conditions. 16S rRNA gene sequence datasets have been submitted to the Sequence Read Archive (SRA) under accession number SRP109130. The Gold Study and Project submission ID number in Joint Genome Institute IMG/M for the metagenome is Gs0047444 and Gp0055765.

Original languageEnglish
Pages (from-to)1845
JournalFrontiers in Microbiology
Volume8
DOIs
Publication statusPublished - 2017
Externally publishedYes

Fingerprint

Benzene
Oils
Isotopes
Metagenomics
Water
Hydrocarbons
rRNA Genes
Thauera
Chromatiaceae
Metagenome
Polycyclic Compounds
Oxygenases
Gold
Genes
Canada
naphthalene
Salts
Joints
Metals
Genome

Keywords

  • Metagenomics

ASJC Scopus subject areas

  • Biotechnology

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)

Cite this

Rochman, F. F., Sheremet, A., Tamas, I., Saidi-Mehrabad, A., Kim, J-J., Dong, X., ... Dunfield, P. F. (2017). Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond. Frontiers in Microbiology , 8, 1845. https://doi.org/10.3389/fmicb.2017.01845

Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond. / Rochman, Fauziah F; Sheremet, Andriy; Tamas, Ivica; Saidi-Mehrabad, Alireza; Kim, Joong-Jae; Dong, Xiaoli; Sensen, Christoph W; Gieg, Lisa M; Dunfield, Peter F.

In: Frontiers in Microbiology , Vol. 8, 2017, p. 1845.

Research output: Contribution to journalArticleResearchpeer-review

Rochman, FF, Sheremet, A, Tamas, I, Saidi-Mehrabad, A, Kim, J-J, Dong, X, Sensen, CW, Gieg, LM & Dunfield, PF 2017, 'Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond' Frontiers in Microbiology , vol. 8, pp. 1845. https://doi.org/10.3389/fmicb.2017.01845
Rochman, Fauziah F ; Sheremet, Andriy ; Tamas, Ivica ; Saidi-Mehrabad, Alireza ; Kim, Joong-Jae ; Dong, Xiaoli ; Sensen, Christoph W ; Gieg, Lisa M ; Dunfield, Peter F. / Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond. In: Frontiers in Microbiology . 2017 ; Vol. 8. pp. 1845.
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AU - Kim, Joong-Jae

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