Assessment of skin permeability to topically applied drugs by skin impedance and admittance

Simon Schwingenschuh, Hermann Scharfetter, Orjan G. Martinsen, Beate Boulgaropoulos, Thomas Augustin, Katrin I. Tiffner, Christian Dragatin, Reingard Raml, Christian Hoefferer, Eva Christina Prandl, Frank Sinner, Martin Hajnsek

Research output: Contribution to journalArticle

Abstract

Objective: Pharmacokinetic and pharmacodynamic studies of topically applied drugs are commonly performed by sampling of interstitial fluid with dermal open flow microperfusion and subsequent analysis of the samples. However, the reliability of results from the measured concentration-time profile of the penetrating drug suffers from highly variable skin permeability to topically applied drugs that is mainly caused by inter- and intra-subject variations of the stratum corneum. Thus, statistically significant results can only be achieved by performing high numbers of experiments. To reduce the expenditures needed for such high experiment numbers we aimed to assess the correlation between skin permeability and skin impedance/skin admittance. Approach: We performed an ex vivo drug penetration study with human skin, based on the hypothesis that inter-subject variations of the respective concentration-time profiles can be correlated with variations of the passive electrical properties of the skin. Therefore, skin impedance and skin admittance were related to the skin permeability to the model drug Clobetasol-17-proprionate. Main results: The measured low frequency skin impedance and the skin admittance correlated linearly with the drug concentration-time profiles from dermal sampling. Significance: Skin permeability can be assessed by measuring the passive electrical properties of the skin, which enables correction of skin permeability variations. This allows reduction of experiment numbers in future pharmacokinetic and pharmacodynamic studies with human skin ex vivo and in vivo and leads to diminished study costs.

Original languageEnglish
Pages (from-to)N138-N150
JournalPhysiological measurement
Volume38
Issue number11
DOIs
Publication statusPublished - 1 Nov 2017

Keywords

  • open flow microperfusion
  • pharmacodynamics
  • pharmacokinetics
  • skin admittance
  • skin impedance
  • skin penetration
  • topical drugs

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Biomedical Engineering
  • Physiology (medical)

Fields of Expertise

  • Human- & Biotechnology

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  • Cite this

    Schwingenschuh, S., Scharfetter, H., Martinsen, O. G., Boulgaropoulos, B., Augustin, T., Tiffner, K. I., ... Hajnsek, M. (2017). Assessment of skin permeability to topically applied drugs by skin impedance and admittance. Physiological measurement, 38(11), N138-N150. https://doi.org/10.1088/1361-6579/aa904e