Separation of active laccases from Pleurotus sapidus culture supernatant using aqueous two-phase systems in centrifugal partition chromatography

C. Schwienheer, A. Prinz, T. Zeiner, J. Merz

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

For the production of bio active compounds, e.g., active enzymes or antibodies, a conserved purification process with a minimum loss of active compounds is necessary. In centrifugal partition chromatography (CPC), the separation effect is based on the different distribution of the components to be separated between two immiscible liquid phases. Thereby, one liquid phase is kept stationary in chambers by a centrifugal field and the mobile phase is pumped through via connecting ducts. Aqueous two phase systems (ATPS) are known to provide benign conditions for biochemical products and seem to be promising when used in CPC for purification tasks. However, it is not known if active biochemical compounds can "survive" the conditions in a CPC where strong shear forces can occur due to the two-phasic flow under centrifugal forces. Therefore, this aspect has been faced within this study by the separation of active laccases from a fermentation broth of Pleurotus sapidus. After selecting a suitable ATPS and operating conditions, the activity yield was calculated and the preservation of the active enzymes could be observed. Therefore, CPC could be shown as potentially suitable for the purification of bio-active compounds.

Originalspracheenglisch
Seiten (von - bis)1-7
Seitenumfang7
FachzeitschriftJournal of chromatography / B
Jahrgang1002
DOIs
PublikationsstatusVeröffentlicht - 1 Okt 2015

Fingerprint

Pleurotus
Laccase
Chromatography
Purification
Liquids
Enzymes
Ducts
Fermentation
Antibodies

Schlagwörter

    ASJC Scopus subject areas

    • !!Biochemistry
    • Analytische Chemie
    • Zellbiologie
    • !!Clinical Biochemistry
    • !!Medicine(all)

    Dies zitieren

    Separation of active laccases from Pleurotus sapidus culture supernatant using aqueous two-phase systems in centrifugal partition chromatography. / Schwienheer, C.; Prinz, A.; Zeiner, T.; Merz, J.

    in: Journal of chromatography / B, Jahrgang 1002, 01.10.2015, S. 1-7.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    @article{5966166df77f4c39858f9e3bfa2b9b78,
    title = "Separation of active laccases from Pleurotus sapidus culture supernatant using aqueous two-phase systems in centrifugal partition chromatography",
    abstract = "For the production of bio active compounds, e.g., active enzymes or antibodies, a conserved purification process with a minimum loss of active compounds is necessary. In centrifugal partition chromatography (CPC), the separation effect is based on the different distribution of the components to be separated between two immiscible liquid phases. Thereby, one liquid phase is kept stationary in chambers by a centrifugal field and the mobile phase is pumped through via connecting ducts. Aqueous two phase systems (ATPS) are known to provide benign conditions for biochemical products and seem to be promising when used in CPC for purification tasks. However, it is not known if active biochemical compounds can {"}survive{"} the conditions in a CPC where strong shear forces can occur due to the two-phasic flow under centrifugal forces. Therefore, this aspect has been faced within this study by the separation of active laccases from a fermentation broth of Pleurotus sapidus. After selecting a suitable ATPS and operating conditions, the activity yield was calculated and the preservation of the active enzymes could be observed. Therefore, CPC could be shown as potentially suitable for the purification of bio-active compounds.",
    keywords = "Activity preservation, Aqueous two-phase systems (ATPS), Centrifugal partition chromatography (CPC), Downstream processing, Laccase from Pleurotus sapidus",
    author = "C. Schwienheer and A. Prinz and T. Zeiner and J. Merz",
    year = "2015",
    month = "10",
    day = "1",
    doi = "10.1016/j.jchromb.2015.07.050",
    language = "English",
    volume = "1002",
    pages = "1--7",
    journal = "Journal of chromatography / B",
    issn = "1570-0232",
    publisher = "Elsevier B.V.",

    }

    TY - JOUR

    T1 - Separation of active laccases from Pleurotus sapidus culture supernatant using aqueous two-phase systems in centrifugal partition chromatography

    AU - Schwienheer, C.

    AU - Prinz, A.

    AU - Zeiner, T.

    AU - Merz, J.

    PY - 2015/10/1

    Y1 - 2015/10/1

    N2 - For the production of bio active compounds, e.g., active enzymes or antibodies, a conserved purification process with a minimum loss of active compounds is necessary. In centrifugal partition chromatography (CPC), the separation effect is based on the different distribution of the components to be separated between two immiscible liquid phases. Thereby, one liquid phase is kept stationary in chambers by a centrifugal field and the mobile phase is pumped through via connecting ducts. Aqueous two phase systems (ATPS) are known to provide benign conditions for biochemical products and seem to be promising when used in CPC for purification tasks. However, it is not known if active biochemical compounds can "survive" the conditions in a CPC where strong shear forces can occur due to the two-phasic flow under centrifugal forces. Therefore, this aspect has been faced within this study by the separation of active laccases from a fermentation broth of Pleurotus sapidus. After selecting a suitable ATPS and operating conditions, the activity yield was calculated and the preservation of the active enzymes could be observed. Therefore, CPC could be shown as potentially suitable for the purification of bio-active compounds.

    AB - For the production of bio active compounds, e.g., active enzymes or antibodies, a conserved purification process with a minimum loss of active compounds is necessary. In centrifugal partition chromatography (CPC), the separation effect is based on the different distribution of the components to be separated between two immiscible liquid phases. Thereby, one liquid phase is kept stationary in chambers by a centrifugal field and the mobile phase is pumped through via connecting ducts. Aqueous two phase systems (ATPS) are known to provide benign conditions for biochemical products and seem to be promising when used in CPC for purification tasks. However, it is not known if active biochemical compounds can "survive" the conditions in a CPC where strong shear forces can occur due to the two-phasic flow under centrifugal forces. Therefore, this aspect has been faced within this study by the separation of active laccases from a fermentation broth of Pleurotus sapidus. After selecting a suitable ATPS and operating conditions, the activity yield was calculated and the preservation of the active enzymes could be observed. Therefore, CPC could be shown as potentially suitable for the purification of bio-active compounds.

    KW - Activity preservation

    KW - Aqueous two-phase systems (ATPS)

    KW - Centrifugal partition chromatography (CPC)

    KW - Downstream processing

    KW - Laccase from Pleurotus sapidus

    UR - http://www.scopus.com/inward/record.url?scp=84939614388&partnerID=8YFLogxK

    U2 - 10.1016/j.jchromb.2015.07.050

    DO - 10.1016/j.jchromb.2015.07.050

    M3 - Article

    VL - 1002

    SP - 1

    EP - 7

    JO - Journal of chromatography / B

    JF - Journal of chromatography / B

    SN - 1570-0232

    ER -