Ion exchange membrane adsorption of bovine serum albumin-Impact of operating and buffer conditions on breakthrough curves

Peter van Beijeren, Peter Kreis, Tim Zeiner

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

The relevant phenomena underlying the adsorption of proteins on ion exchange membranes with grafted polymer layers are not well understood yet. In this work the influence of operating and buffer conditions on the adsorption of bovine serum albumin (BSA) on anion and cation exchange membrane adsorbers is systematically investigated in order to find indications on the relevant mechanisms. The experimental results obtained at different buffer conditions are analyzed using the steric mass action model.Large differences were observed between the adsorption behavior of BSA on anion and cation exchange membranes. On the anion exchange membranes the dynamic binding capacity at 10% breakthrough was independent of the applied flow rate and decreased with increasing salt concentration. This in contrast to the cation exchange membranes, for which the dynamic binding capacity was strongly dependent on the flow rate and for which an optimum in binding capacity was observed at an intermediate salt concentration. Especially, the results obtained on cation exchange suggest a strong role of non-ideal effects such as steric hindrance and electrostatic repulsion in membrane adsorption. An improved understanding of these effects is required for further optimization of membrane adsorber materials.

Originalspracheenglisch
Seiten (von - bis)568-576
Seitenumfang9
FachzeitschriftJournal of membrane science
Jahrgang415-416
DOIs
PublikationsstatusVeröffentlicht - 1 Okt 2012

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Ion exchange membranes
Ion Exchange
Bovine Serum Albumin
albumins
serums
Adsorption
Buffers
buffers
Ion exchange
membranes
Membranes
adsorption
Cations
curves
Positive ions
Anions
ions
Negative ions
cations
anions

Schlagwörter

    ASJC Scopus subject areas

    • !!Physical and Theoretical Chemistry
    • !!Materials Science(all)
    • !!Biochemistry
    • !!Filtration and Separation

    Dies zitieren

    Ion exchange membrane adsorption of bovine serum albumin-Impact of operating and buffer conditions on breakthrough curves. / van Beijeren, Peter; Kreis, Peter; Zeiner, Tim.

    in: Journal of membrane science, Jahrgang 415-416, 01.10.2012, S. 568-576.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

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