Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: Reaction intermediates revealed by crystallography and mass spectrometry

Barbara S. Lang, Antonius C.F. Gorren, Gustav Oberdorfer, M. Verena Wenzl, Cristina M. Furdui, Leslie B. Poole, Bernd Mayer, Karl Gruber

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Aldehyde dehydrogenase-2 (ALDH2) catalyzes bioactivation of glyceryl trinitrate (GTN) resulting in vasodilation. The exact mechanism is still unclear. Results: Structures of ALDH2 in complex with GTN and of a thionitrate intermediate were obtained. Conclusion: The structures represent snapshots of the first reaction step of GTN bioactivation by ALDH2. Significance: The results provide new insight into the mechanism of vascular GTN bioactivation by ALDH2.

Original languageEnglish
Pages (from-to)38124-38134
Number of pages11
JournalJournal of Biological Chemistry
Volume287
Issue number45
DOIs
Publication statusPublished - 2 Nov 2012

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Reaction intermediates
Aldehyde Dehydrogenase
Crystallography
Nitroglycerin
Mass spectrometry
Blood Vessels
Mass Spectrometry
Vasodilation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2 : Reaction intermediates revealed by crystallography and mass spectrometry. / Lang, Barbara S.; Gorren, Antonius C.F.; Oberdorfer, Gustav; Wenzl, M. Verena; Furdui, Cristina M.; Poole, Leslie B.; Mayer, Bernd; Gruber, Karl.

In: Journal of Biological Chemistry, Vol. 287, No. 45, 02.11.2012, p. 38124-38134.

Research output: Contribution to journalArticleResearchpeer-review

Lang, Barbara S. ; Gorren, Antonius C.F. ; Oberdorfer, Gustav ; Wenzl, M. Verena ; Furdui, Cristina M. ; Poole, Leslie B. ; Mayer, Bernd ; Gruber, Karl. / Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2 : Reaction intermediates revealed by crystallography and mass spectrometry. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 45. pp. 38124-38134.
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