Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: Reaction intermediates revealed by crystallography and mass spectrometry

Barbara S. Lang, Antonius C.F. Gorren, Gustav Oberdorfer, M. Verena Wenzl, Cristina M. Furdui, Leslie B. Poole, Bernd Mayer*, Karl Gruber

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Background: Aldehyde dehydrogenase-2 (ALDH2) catalyzes bioactivation of glyceryl trinitrate (GTN) resulting in vasodilation. The exact mechanism is still unclear. Results: Structures of ALDH2 in complex with GTN and of a thionitrate intermediate were obtained. Conclusion: The structures represent snapshots of the first reaction step of GTN bioactivation by ALDH2. Significance: The results provide new insight into the mechanism of vascular GTN bioactivation by ALDH2.

Original languageEnglish
Pages (from-to)38124-38134
Number of pages11
JournalJournal of Biological Chemistry
Volume287
Issue number45
DOIs
Publication statusPublished - 2 Nov 2012

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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