Translin and Trax differentially regulate telomere-associated transcript homeostasis

Natalia Gomez-Escobar, Nasser Almobadel, Othman Alzahrani, Julia Feichtinger, Vicente Planells-Palop, Zafer Alshehri, Gerhard G Thallinger, Jane A Wakeman, Ramsay J McFarlane

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Translin and Trax proteins are highly conserved nucleic acid binding proteins that have been implicated in RNA regulation in a range of biological processes including tRNA processing, RNA interference, microRNA degradation during oncogenesis, spermatogenesis and neuronal regulation. Here, we explore the function of this paralogue pair of proteins in the fission yeast. Using transcript analysis we demonstrate a reciprocal mechanism for control of telomere-associated transcripts. Mutation of tfx1+ (Trax) elevates transcript levels from silenced sub-telomeric regions of the genome, but not other silenced regions, such as the peri-centromeric heterochromatin. In the case of some sub-telomeric transcripts, but not all, this elevation is dependent on the Trax paralogue, Tsn1 (Translin). In a reciprocal fashion, Tsn1 (Translin) serves to repress levels of transcripts (TERRAs) from the telomeric repeats, whereas Tfx1 serves to maintain these elevated levels. This reveals a novel mechanism for the regulation of telomeric transcripts. We extend this to demonstrate that human Translin and Trax also control telomere-associated transcript levels in human cells in a telomere-specific fashion.

Original languageEnglish
Pages (from-to)33809-20
Number of pages12
JournalOncoTarget
Volume7
Issue number23
DOIs
Publication statusPublished - 7 Jun 2016

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Telomere
Homeostasis
Schizosaccharomyces pombe Proteins
Biological Phenomena
Heterochromatin
Spermatogenesis
RNA Interference
Transfer RNA
MicroRNAs
Nucleic Acids
Carrier Proteins
Carcinogenesis
Genome
RNA
Mutation
Proteins

Keywords

  • Journal Article

Cite this

Gomez-Escobar, N., Almobadel, N., Alzahrani, O., Feichtinger, J., Planells-Palop, V., Alshehri, Z., ... McFarlane, R. J. (2016). Translin and Trax differentially regulate telomere-associated transcript homeostasis. OncoTarget , 7(23), 33809-20. https://doi.org/10.18632/oncotarget.9278

Translin and Trax differentially regulate telomere-associated transcript homeostasis. / Gomez-Escobar, Natalia; Almobadel, Nasser; Alzahrani, Othman; Feichtinger, Julia; Planells-Palop, Vicente; Alshehri, Zafer; Thallinger, Gerhard G; Wakeman, Jane A; McFarlane, Ramsay J.

In: OncoTarget , Vol. 7, No. 23, 07.06.2016, p. 33809-20.

Research output: Contribution to journalArticleResearchpeer-review

Gomez-Escobar, N, Almobadel, N, Alzahrani, O, Feichtinger, J, Planells-Palop, V, Alshehri, Z, Thallinger, GG, Wakeman, JA & McFarlane, RJ 2016, 'Translin and Trax differentially regulate telomere-associated transcript homeostasis' OncoTarget , vol. 7, no. 23, pp. 33809-20. https://doi.org/10.18632/oncotarget.9278
Gomez-Escobar N, Almobadel N, Alzahrani O, Feichtinger J, Planells-Palop V, Alshehri Z et al. Translin and Trax differentially regulate telomere-associated transcript homeostasis. OncoTarget . 2016 Jun 7;7(23):33809-20. https://doi.org/10.18632/oncotarget.9278
Gomez-Escobar, Natalia ; Almobadel, Nasser ; Alzahrani, Othman ; Feichtinger, Julia ; Planells-Palop, Vicente ; Alshehri, Zafer ; Thallinger, Gerhard G ; Wakeman, Jane A ; McFarlane, Ramsay J. / Translin and Trax differentially regulate telomere-associated transcript homeostasis. In: OncoTarget . 2016 ; Vol. 7, No. 23. pp. 33809-20.
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