Time-Resolved Fluorescence HPLC Detection Using Semiconductor Light Sources: Principles and Applications

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The simultaneous static and time-resolved fluorescence detection in high performance liquid chromatography (HPLC) systems using time-correlated single photon counting (TCSPC) and modulation techniques is a very recent topic in the area of analytical and physical chemistry. Whereas most commercial systems use simple intensity detection in fluorescence systems or fluorescence spectra with very low sensitivity we use real-time time-resolved fluorescence counting with on-the-fly data evaluation and display like a typical chromatogram. The static measurement is the sum of counts in the time interval. Both results are independent and characteristic for the fluorescent analytes. Additionally they are of equal sensitivity. In order to achieve ns resolution modern semiconductor light sources (nowadays available from 235 nm to MIR) are used. They are fast, small, low cost, low energy consuming, durable, and reliable (no maintenance necessary). Modern electronics allow a setup in typical HPLC component size in contrast to older complicated lamp or laser applications. For the measuring procedure no adjustment or calibration for the chromatogram is necessary. A rapid modification for new applications, such as new developments for legal regulations, is possible due to the flexible modular design. The application is patent pending: DE 102010016801 A1.
Original languageEnglish
Pages (from-to)175-182
JournalChemical engineering & technology
Volume39
DOIs
Publication statusPublished - 1 May 2016

Keywords

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry

    Fields of Expertise

    • Advanced Materials Science

    Treatment code (Nähere Zuordnung)

    • Experimental

    Cite this

    Time-Resolved Fluorescence HPLC Detection Using Semiconductor Light Sources: Principles and Applications. / Landgraf, Stephan.

    In: Chemical engineering & technology, Vol. 39, 01.05.2016, p. 175-182.

    Research output: Contribution to journalArticleResearchpeer-review

    @article{c48369aa86ae4b12bfa716e282fd152e,
    title = "Time-Resolved Fluorescence HPLC Detection Using Semiconductor Light Sources: Principles and Applications",
    abstract = "The simultaneous static and time-resolved fluorescence detection in high performance liquid chromatography (HPLC) systems using time-correlated single photon counting (TCSPC) and modulation techniques is a very recent topic in the area of analytical and physical chemistry. Whereas most commercial systems use simple intensity detection in fluorescence systems or fluorescence spectra with very low sensitivity we use real-time time-resolved fluorescence counting with on-the-fly data evaluation and display like a typical chromatogram. The static measurement is the sum of counts in the time interval. Both results are independent and characteristic for the fluorescent analytes. Additionally they are of equal sensitivity. In order to achieve ns resolution modern semiconductor light sources (nowadays available from 235 nm to MIR) are used. They are fast, small, low cost, low energy consuming, durable, and reliable (no maintenance necessary). Modern electronics allow a setup in typical HPLC component size in contrast to older complicated lamp or laser applications. For the measuring procedure no adjustment or calibration for the chromatogram is necessary. A rapid modification for new applications, such as new developments for legal regulations, is possible due to the flexible modular design. The application is patent pending: DE 102010016801 A1.",
    keywords = "HPLC , Fluorescence, Time-Resolved, Semiconductor Light Sources, TCSPC",
    author = "Stephan Landgraf",
    year = "2016",
    month = "5",
    day = "1",
    doi = "DOI: 10.1002/ceat.201500152",
    language = "English",
    volume = "39",
    pages = "175--182",
    journal = "Chemical engineering & technology",
    issn = "0930-7516",
    publisher = "Wiley-VCH",

    }

    TY - JOUR

    T1 - Time-Resolved Fluorescence HPLC Detection Using Semiconductor Light Sources: Principles and Applications

    AU - Landgraf, Stephan

    PY - 2016/5/1

    Y1 - 2016/5/1

    N2 - The simultaneous static and time-resolved fluorescence detection in high performance liquid chromatography (HPLC) systems using time-correlated single photon counting (TCSPC) and modulation techniques is a very recent topic in the area of analytical and physical chemistry. Whereas most commercial systems use simple intensity detection in fluorescence systems or fluorescence spectra with very low sensitivity we use real-time time-resolved fluorescence counting with on-the-fly data evaluation and display like a typical chromatogram. The static measurement is the sum of counts in the time interval. Both results are independent and characteristic for the fluorescent analytes. Additionally they are of equal sensitivity. In order to achieve ns resolution modern semiconductor light sources (nowadays available from 235 nm to MIR) are used. They are fast, small, low cost, low energy consuming, durable, and reliable (no maintenance necessary). Modern electronics allow a setup in typical HPLC component size in contrast to older complicated lamp or laser applications. For the measuring procedure no adjustment or calibration for the chromatogram is necessary. A rapid modification for new applications, such as new developments for legal regulations, is possible due to the flexible modular design. The application is patent pending: DE 102010016801 A1.

    AB - The simultaneous static and time-resolved fluorescence detection in high performance liquid chromatography (HPLC) systems using time-correlated single photon counting (TCSPC) and modulation techniques is a very recent topic in the area of analytical and physical chemistry. Whereas most commercial systems use simple intensity detection in fluorescence systems or fluorescence spectra with very low sensitivity we use real-time time-resolved fluorescence counting with on-the-fly data evaluation and display like a typical chromatogram. The static measurement is the sum of counts in the time interval. Both results are independent and characteristic for the fluorescent analytes. Additionally they are of equal sensitivity. In order to achieve ns resolution modern semiconductor light sources (nowadays available from 235 nm to MIR) are used. They are fast, small, low cost, low energy consuming, durable, and reliable (no maintenance necessary). Modern electronics allow a setup in typical HPLC component size in contrast to older complicated lamp or laser applications. For the measuring procedure no adjustment or calibration for the chromatogram is necessary. A rapid modification for new applications, such as new developments for legal regulations, is possible due to the flexible modular design. The application is patent pending: DE 102010016801 A1.

    KW - HPLC

    KW - Fluorescence

    KW - Time-Resolved

    KW - Semiconductor Light Sources

    KW - TCSPC

    U2 - DOI: 10.1002/ceat.201500152

    DO - DOI: 10.1002/ceat.201500152

    M3 - Article

    VL - 39

    SP - 175

    EP - 182

    JO - Chemical engineering & technology

    JF - Chemical engineering & technology

    SN - 0930-7516

    ER -