The novel lipopeptide poaeamide of the endophyte Pseudomonas poae RE*1-1-14 is involved in pathogen suppression and root colonization

Christin Zachow, G Jahanshah, I de Bruijn, C Song, F Ianni, Z Pataj, H Gerhardt, I Pianet, M Lämmerhofer, Gabriele Berg, H Gross, JM Raaijmakers

Research output: Contribution to journalArticle

Abstract

Endophytic Pseudomonas poae strain RE*1-1-14 was originally isolated from internal root tissue of sugar beet plants and shown to suppress growth of the fungal pathogen Rhizoctonia solani both in vitro and in the field. To identify genes involved in its biocontrol activity, RE*1-1-14 random mutagenesis and sequencing led to the identification of a nonribosomal peptide synthetase (NRPS) gene cluster predicted to encode a lipopeptide (LP) with a 10-amino-acid peptide moiety. The two unlinked gene clusters consisted of three NRPS genes, designated poaA (cluster 1) and poaB and poaC (cluster 2), spanning approximately 33.7 kb. In silico analysis followed by chemical analyses revealed that the encoded LP, designated poaeamide, is a structurally new member of the orfamide family. Poaeamide inhibited mycelial growth of R. solani and different oomycetes, including Phytophthora capsici, P. infestans, and …
Original languageEnglish
Pages (from-to)800
Number of pages810
JournalMolecular Plant-Microbe Interactions
Volume28(7)
DOIs
Publication statusPublished - 2015

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