The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori

Ingeborg Klymiuk, Ceren Bilgilier, Alexander Stadlmann, Jakob Thannesberger, Marie-Theres Kastner, Christoph Högenauer, Andreas Püspök, Susanne Biowski-Frotz, Christiane Schrutka-Kölbl, Gerhard G Thallinger, Christoph Steininger

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The human gastric lumen is one of the most hostile environments of the human body suspected to be sterile until the discovery of Helicobacter pylori (H.p.). State of the art next generation sequencing technologies multiply the knowledge on H.p. functional genomics as well as on the colonization of supposed sterile human environments like the gastric habitat. Here we studied in a prospective, multicenter, clinical trial the 16S rRNA gene amplicon based bacterial microbiome in a total of 30 homogenized and frozen gastric biopsy samples from eight geographic locations. The evaluation of the samples for H.p. infection status was done by histopathology and a specific PCR assay. CagA status was determined by a CagA-specific PCR assay. Patients were grouped accordingly as H.p.-negative, H.p.-positive but CagA-negative and H.p.-positive and CagA-positive (n = 10, respectively). Here we show that H.p. infection of the gastric habitat dominates the gastric microbiota in most patients and is associated with a significant decrease of the microbial alpha diversity from H.p. negative to H.p. positive with CagA as a considerable factor. The genera Actinomyces, Granulicatella, Veillonella, Fusobacterium, Neisseria, Helicobacter, Streptococcus, and Prevotella are significantly different between the H.p.-positive and H.p.-negative sample groups. Differences in microbiota found between CagA-positive and CagA-negative patients were not statistically significant and need to be re-evaluated in larger sample cohorts. In conclusion, H.p. infection dominates the gastric microbiome in a multicentre cohort of patients with varying diagnoses.

Original languageEnglish
Article number2508
JournalFrontiers in Microbiology
Volume8
DOIs
Publication statusPublished - 14 Dec 2017

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Microbiota
Helicobacter pylori
Stomach
Infection
Helicobacter Infections
Ecosystem
Veillonella
Fusobacterium
Prevotella
Helicobacter
Neisseria
Actinomyces
Geographic Locations
Polymerase Chain Reaction
Genomics
Streptococcus
rRNA Genes
Human Body
Multicenter Studies
Clinical Trials

Keywords

  • Journal Article

Fields of Expertise

  • Human- & Biotechnology
  • Information, Communication & Computing

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)
  • Experimental

Cooperations

  • BioTechMed-Graz

Cite this

Klymiuk, I., Bilgilier, C., Stadlmann, A., Thannesberger, J., Kastner, M-T., Högenauer, C., ... Steininger, C. (2017). The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori. Frontiers in Microbiology , 8, [2508]. https://doi.org/10.3389/fmicb.2017.02508

The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori. / Klymiuk, Ingeborg; Bilgilier, Ceren; Stadlmann, Alexander; Thannesberger, Jakob; Kastner, Marie-Theres; Högenauer, Christoph; Püspök, Andreas; Biowski-Frotz, Susanne; Schrutka-Kölbl, Christiane; Thallinger, Gerhard G; Steininger, Christoph.

In: Frontiers in Microbiology , Vol. 8, 2508, 14.12.2017.

Research output: Contribution to journalArticleResearchpeer-review

Klymiuk, I, Bilgilier, C, Stadlmann, A, Thannesberger, J, Kastner, M-T, Högenauer, C, Püspök, A, Biowski-Frotz, S, Schrutka-Kölbl, C, Thallinger, GG & Steininger, C 2017, 'The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori' Frontiers in Microbiology , vol. 8, 2508. https://doi.org/10.3389/fmicb.2017.02508
Klymiuk I, Bilgilier C, Stadlmann A, Thannesberger J, Kastner M-T, Högenauer C et al. The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori. Frontiers in Microbiology . 2017 Dec 14;8. 2508. https://doi.org/10.3389/fmicb.2017.02508
Klymiuk, Ingeborg ; Bilgilier, Ceren ; Stadlmann, Alexander ; Thannesberger, Jakob ; Kastner, Marie-Theres ; Högenauer, Christoph ; Püspök, Andreas ; Biowski-Frotz, Susanne ; Schrutka-Kölbl, Christiane ; Thallinger, Gerhard G ; Steininger, Christoph. / The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori. In: Frontiers in Microbiology . 2017 ; Vol. 8.
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