Subpallial Enhancer Transgenic Lines: a Data and Tool Resource to Study Transcriptional Regulation of GABAergic Cell Fate

Shanni N Silberberg, Leila Taher, Susan Lindtner, Magnus Sandberg, Alex S Nord, Daniel Vogt, Gabriel L Mckinsey, Renee Hoch, Kartik Pattabiraman, Dongji Zhang, Jose L Ferran, Aleksandar Rajkovic, Olga Golonzhka, Carol Kim, Hongkui Zeng, Luis Puelles, Axel Visel, John L R Rubenstein

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Elucidating the transcriptional circuitry controlling forebrain development requires an understanding of enhancer activity and regulation. We generated stable transgenic mouse lines that express CreERT2 and GFP from ten different enhancer elements with activity in distinct domains within the embryonic basal ganglia. We used these unique tools to generate a comprehensive regional fate map of the mouse subpallium, including sources for specific subtypes of amygdala neurons. We then focused on deciphering transcriptional mechanisms that control enhancer activity. Using machine-learning computations, in vivo chromosomal occupancy of 13 transcription factors that regulate subpallial patterning and differentiation and analysis of enhancer activity in Dlx1/2 and Lhx6 mutants, we elucidated novel molecular mechanisms that regulate region-specific enhancer activity in the developing brain. Thus, these subpallial enhancer transgenic lines are data and tool resources to study transcriptional regulation of GABAergic cell fate.

Original languageEnglish
Pages (from-to)59-74
Number of pages16
JournalNeuron
Volume92
Issue number1
DOIs
Publication statusPublished - 5 Oct 2016
Externally publishedYes

Fingerprint

Prosencephalon
Amygdala
Basal Ganglia
Transgenic Mice
Transcription Factors
Neurons
Brain
Machine Learning

Keywords

  • Animals
  • Basal Ganglia/growth & development
  • Cell Differentiation/genetics
  • Enhancer Elements, Genetic/genetics
  • GABAergic Neurons/cytology
  • Gene Expression Regulation, Developmental/genetics
  • Homeodomain Proteins/genetics
  • LIM-Homeodomain Proteins/genetics
  • Mice
  • Mice, Transgenic
  • Nerve Tissue Proteins/genetics
  • Transcription Factors/genetics

Cite this

Subpallial Enhancer Transgenic Lines : a Data and Tool Resource to Study Transcriptional Regulation of GABAergic Cell Fate. / Silberberg, Shanni N; Taher, Leila; Lindtner, Susan; Sandberg, Magnus; Nord, Alex S; Vogt, Daniel; Mckinsey, Gabriel L; Hoch, Renee; Pattabiraman, Kartik; Zhang, Dongji; Ferran, Jose L; Rajkovic, Aleksandar; Golonzhka, Olga; Kim, Carol; Zeng, Hongkui; Puelles, Luis; Visel, Axel; Rubenstein, John L R.

In: Neuron, Vol. 92, No. 1, 05.10.2016, p. 59-74.

Research output: Contribution to journalArticleResearchpeer-review

Silberberg, SN, Taher, L, Lindtner, S, Sandberg, M, Nord, AS, Vogt, D, Mckinsey, GL, Hoch, R, Pattabiraman, K, Zhang, D, Ferran, JL, Rajkovic, A, Golonzhka, O, Kim, C, Zeng, H, Puelles, L, Visel, A & Rubenstein, JLR 2016, 'Subpallial Enhancer Transgenic Lines: a Data and Tool Resource to Study Transcriptional Regulation of GABAergic Cell Fate' Neuron, vol. 92, no. 1, pp. 59-74. https://doi.org/10.1016/j.neuron.2016.09.027
Silberberg, Shanni N ; Taher, Leila ; Lindtner, Susan ; Sandberg, Magnus ; Nord, Alex S ; Vogt, Daniel ; Mckinsey, Gabriel L ; Hoch, Renee ; Pattabiraman, Kartik ; Zhang, Dongji ; Ferran, Jose L ; Rajkovic, Aleksandar ; Golonzhka, Olga ; Kim, Carol ; Zeng, Hongkui ; Puelles, Luis ; Visel, Axel ; Rubenstein, John L R. / Subpallial Enhancer Transgenic Lines : a Data and Tool Resource to Study Transcriptional Regulation of GABAergic Cell Fate. In: Neuron. 2016 ; Vol. 92, No. 1. pp. 59-74.
@article{0dded1f20a9646e984894aa7fdb4a12e,
title = "Subpallial Enhancer Transgenic Lines: a Data and Tool Resource to Study Transcriptional Regulation of GABAergic Cell Fate",
abstract = "Elucidating the transcriptional circuitry controlling forebrain development requires an understanding of enhancer activity and regulation. We generated stable transgenic mouse lines that express CreERT2 and GFP from ten different enhancer elements with activity in distinct domains within the embryonic basal ganglia. We used these unique tools to generate a comprehensive regional fate map of the mouse subpallium, including sources for specific subtypes of amygdala neurons. We then focused on deciphering transcriptional mechanisms that control enhancer activity. Using machine-learning computations, in vivo chromosomal occupancy of 13 transcription factors that regulate subpallial patterning and differentiation and analysis of enhancer activity in Dlx1/2 and Lhx6 mutants, we elucidated novel molecular mechanisms that regulate region-specific enhancer activity in the developing brain. Thus, these subpallial enhancer transgenic lines are data and tool resources to study transcriptional regulation of GABAergic cell fate.",
keywords = "Animals, Basal Ganglia/growth & development, Cell Differentiation/genetics, Enhancer Elements, Genetic/genetics, GABAergic Neurons/cytology, Gene Expression Regulation, Developmental/genetics, Homeodomain Proteins/genetics, LIM-Homeodomain Proteins/genetics, Mice, Mice, Transgenic, Nerve Tissue Proteins/genetics, Transcription Factors/genetics",
author = "Silberberg, {Shanni N} and Leila Taher and Susan Lindtner and Magnus Sandberg and Nord, {Alex S} and Daniel Vogt and Mckinsey, {Gabriel L} and Renee Hoch and Kartik Pattabiraman and Dongji Zhang and Ferran, {Jose L} and Aleksandar Rajkovic and Olga Golonzhka and Carol Kim and Hongkui Zeng and Luis Puelles and Axel Visel and Rubenstein, {John L R}",
note = "Copyright {\circledC} 2016 Elsevier Inc. All rights reserved.",
year = "2016",
month = "10",
day = "5",
doi = "10.1016/j.neuron.2016.09.027",
language = "English",
volume = "92",
pages = "59--74",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - Subpallial Enhancer Transgenic Lines

T2 - a Data and Tool Resource to Study Transcriptional Regulation of GABAergic Cell Fate

AU - Silberberg, Shanni N

AU - Taher, Leila

AU - Lindtner, Susan

AU - Sandberg, Magnus

AU - Nord, Alex S

AU - Vogt, Daniel

AU - Mckinsey, Gabriel L

AU - Hoch, Renee

AU - Pattabiraman, Kartik

AU - Zhang, Dongji

AU - Ferran, Jose L

AU - Rajkovic, Aleksandar

AU - Golonzhka, Olga

AU - Kim, Carol

AU - Zeng, Hongkui

AU - Puelles, Luis

AU - Visel, Axel

AU - Rubenstein, John L R

N1 - Copyright © 2016 Elsevier Inc. All rights reserved.

PY - 2016/10/5

Y1 - 2016/10/5

N2 - Elucidating the transcriptional circuitry controlling forebrain development requires an understanding of enhancer activity and regulation. We generated stable transgenic mouse lines that express CreERT2 and GFP from ten different enhancer elements with activity in distinct domains within the embryonic basal ganglia. We used these unique tools to generate a comprehensive regional fate map of the mouse subpallium, including sources for specific subtypes of amygdala neurons. We then focused on deciphering transcriptional mechanisms that control enhancer activity. Using machine-learning computations, in vivo chromosomal occupancy of 13 transcription factors that regulate subpallial patterning and differentiation and analysis of enhancer activity in Dlx1/2 and Lhx6 mutants, we elucidated novel molecular mechanisms that regulate region-specific enhancer activity in the developing brain. Thus, these subpallial enhancer transgenic lines are data and tool resources to study transcriptional regulation of GABAergic cell fate.

AB - Elucidating the transcriptional circuitry controlling forebrain development requires an understanding of enhancer activity and regulation. We generated stable transgenic mouse lines that express CreERT2 and GFP from ten different enhancer elements with activity in distinct domains within the embryonic basal ganglia. We used these unique tools to generate a comprehensive regional fate map of the mouse subpallium, including sources for specific subtypes of amygdala neurons. We then focused on deciphering transcriptional mechanisms that control enhancer activity. Using machine-learning computations, in vivo chromosomal occupancy of 13 transcription factors that regulate subpallial patterning and differentiation and analysis of enhancer activity in Dlx1/2 and Lhx6 mutants, we elucidated novel molecular mechanisms that regulate region-specific enhancer activity in the developing brain. Thus, these subpallial enhancer transgenic lines are data and tool resources to study transcriptional regulation of GABAergic cell fate.

KW - Animals

KW - Basal Ganglia/growth & development

KW - Cell Differentiation/genetics

KW - Enhancer Elements, Genetic/genetics

KW - GABAergic Neurons/cytology

KW - Gene Expression Regulation, Developmental/genetics

KW - Homeodomain Proteins/genetics

KW - LIM-Homeodomain Proteins/genetics

KW - Mice

KW - Mice, Transgenic

KW - Nerve Tissue Proteins/genetics

KW - Transcription Factors/genetics

U2 - 10.1016/j.neuron.2016.09.027

DO - 10.1016/j.neuron.2016.09.027

M3 - Article

VL - 92

SP - 59

EP - 74

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 1

ER -