Studying seed microbiomes

Birgit Wassermann, Daria Rybakova, Eveline Adam, Christin Zachow, Maria Bernhard, Maria Müller, Riccardo Mancinelli, Gabriele Berg*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Recent studies indicate that seed microbiomes affect germination and plant performance. However, the interplay between seed microbiota and plant health is still poorly understood. To get a complete picture of the system, a comprehensive analysis is required, comprising culture-dependent and culture-independent techniques. In this chapter, we provide a combination of methods that are established and optimized for the analysis of the seed microbiome. These include methods to: (1) activate and cultivate dormant seed microbiota, (2) analyze microbiota in germinated seeds (with and without substrate), (3) quantify microbial DNA via real-time PCR, (4) deplete host DNA for amplicon and metagenome analysis, and (5) visualize seed endophytes in microtomed sections using fluorescent in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). A deep understanding of the seed microbiome and its functions can help in developing new seed treatments and breeding strategies for sustainable agriculture.

Original languageEnglish
Title of host publicationThe Plant Microbiome
Subtitle of host publicationMethods and Protocols
EditorsLilia C. Carvalhais, Paul G. Dennis
Place of PublicationNew York, NY
PublisherHumana Press Inc.
Pages1-21
Number of pages21
ISBN (Electronic)978-1-0716-1040-4
ISBN (Print)978-1-0716-1039-8
DOIs
Publication statusPublished - 1 Jan 2021

Publication series

NameMethods in Molecular Biology
Volume2232
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • FISH-CLSM
  • Germination
  • Microbiota
  • Peptide nucleic acid
  • Real-time PCR
  • Seed microbiome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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