Structure and function of the symbiosis partners of the lung lichen (Lobaria pulmonaria L. Hoffm.) analyzed by metaproteomics

T. Schneider, Joao Vieira De Castro Junior, Massimiliano Cardinale, L. Eberl, Martin Grube, Gabriele Berg, K. Riedel

Research output: Contribution to journalArticle

Abstract

Environmental proteomics, also referred to as metaproteomics, is an emerging technology to study the structure and function of microbial communities. Here, we applied semi‐quantitative label‐free proteomics using one‐dimensional gel electrophoresis combined with LC‐MS/MS and normalized spectral counting together with fluorescence in situ hybridization and confocal laser scanning microscopy to characterize the metaproteome of the lung lichen symbiosis Lobaria pulmonaria. In addition to the myco‐ and photobiont, L. pulmonaria harbors proteins from a highly diverse prokaryotic community, which is dominated by Proteobacteria and including also Archaea. While fungal proteins are most dominant (75.4% of all assigned spectra), about the same amount of spectra were assigned to prokaryotic proteins (10%) and to the green algal photobiont (9%). While the latter proteins were found to be mainly associated …
Original languageEnglish
Pages (from-to)2752-2756
JournalProteomics
Issue number13
DOIs
Publication statusPublished - Jul 2011

Fields of Expertise

  • Sonstiges

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