Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts

Ana I. Benítez-Mateos, Bernd Nidetzky, Juan M. Bolivar, Fernando López-Gallego

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Immobilized enzymes have been widely exploited because they work as heterogeneous biocatalysts, allowing their recovery and reutilization and easing the downstream processing once the chemical reactions are completed. Unfortunately, we suffer a lack of analytical methods to characterize those heterogeneous biocatalysts at microscopic and molecular levels with spatio-temporal resolution, which limits their design and optimization. Single-particle studies are vital to optimize the performance of immobilized enzymes in micro/nanoscopic environments. In this Concept article, we review different analytical techniques that address single-particle studies to image the spatial distribution of the enzymes across the solid surfaces, the sub-particle substrate diffusion, the structural integrity and mobility of the immobilized enzymes inside the solid particles, and the pH and O2 internal gradients. From our view, such sub-particle information elicited from single-particle analysis is paramount for the design and fabrication of optimal heterogeneous biocatalyst.

Original languageEnglish
Pages (from-to)654-665
Number of pages12
JournalChemCatChem
Volume10
Issue number4
DOIs
Publication statusPublished - 21 Feb 2018

Fingerprint

Biocatalysts
Immobilized Enzymes
Enzymes
enzymes
Structural integrity
Spatial distribution
Chemical reactions
temporal resolution
Fabrication
Recovery
solid surfaces
integrity
chemical reactions
spatial distribution
Substrates
Processing
recovery
gradients
fabrication
optimization

Keywords

  • heterogeneous biocatalysis
  • microscopy
  • multi-enzymatic systems
  • protein immobilization
  • spectroscopy

ASJC Scopus subject areas

  • Catalysis
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts. / Benítez-Mateos, Ana I.; Nidetzky, Bernd; Bolivar, Juan M.; López-Gallego, Fernando.

In: ChemCatChem, Vol. 10, No. 4, 21.02.2018, p. 654-665.

Research output: Contribution to journalArticleResearchpeer-review

Benítez-Mateos, Ana I. ; Nidetzky, Bernd ; Bolivar, Juan M. ; López-Gallego, Fernando. / Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts. In: ChemCatChem. 2018 ; Vol. 10, No. 4. pp. 654-665.
@article{ddc4e99f2cfd4f1281b17d78a97e0ce3,
title = "Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts",
abstract = "Immobilized enzymes have been widely exploited because they work as heterogeneous biocatalysts, allowing their recovery and reutilization and easing the downstream processing once the chemical reactions are completed. Unfortunately, we suffer a lack of analytical methods to characterize those heterogeneous biocatalysts at microscopic and molecular levels with spatio-temporal resolution, which limits their design and optimization. Single-particle studies are vital to optimize the performance of immobilized enzymes in micro/nanoscopic environments. In this Concept article, we review different analytical techniques that address single-particle studies to image the spatial distribution of the enzymes across the solid surfaces, the sub-particle substrate diffusion, the structural integrity and mobility of the immobilized enzymes inside the solid particles, and the pH and O2 internal gradients. From our view, such sub-particle information elicited from single-particle analysis is paramount for the design and fabrication of optimal heterogeneous biocatalyst.",
keywords = "heterogeneous biocatalysis, microscopy, multi-enzymatic systems, protein immobilization, spectroscopy",
author = "Ben{\'i}tez-Mateos, {Ana I.} and Bernd Nidetzky and Bolivar, {Juan M.} and Fernando L{\'o}pez-Gallego",
year = "2018",
month = "2",
day = "21",
doi = "10.1002/cctc.201701590",
language = "English",
volume = "10",
pages = "654--665",
journal = "ChemCatChem",
issn = "1867-3880",
publisher = "Wiley-VCH",
number = "4",

}

TY - JOUR

T1 - Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts

AU - Benítez-Mateos, Ana I.

AU - Nidetzky, Bernd

AU - Bolivar, Juan M.

AU - López-Gallego, Fernando

PY - 2018/2/21

Y1 - 2018/2/21

N2 - Immobilized enzymes have been widely exploited because they work as heterogeneous biocatalysts, allowing their recovery and reutilization and easing the downstream processing once the chemical reactions are completed. Unfortunately, we suffer a lack of analytical methods to characterize those heterogeneous biocatalysts at microscopic and molecular levels with spatio-temporal resolution, which limits their design and optimization. Single-particle studies are vital to optimize the performance of immobilized enzymes in micro/nanoscopic environments. In this Concept article, we review different analytical techniques that address single-particle studies to image the spatial distribution of the enzymes across the solid surfaces, the sub-particle substrate diffusion, the structural integrity and mobility of the immobilized enzymes inside the solid particles, and the pH and O2 internal gradients. From our view, such sub-particle information elicited from single-particle analysis is paramount for the design and fabrication of optimal heterogeneous biocatalyst.

AB - Immobilized enzymes have been widely exploited because they work as heterogeneous biocatalysts, allowing their recovery and reutilization and easing the downstream processing once the chemical reactions are completed. Unfortunately, we suffer a lack of analytical methods to characterize those heterogeneous biocatalysts at microscopic and molecular levels with spatio-temporal resolution, which limits their design and optimization. Single-particle studies are vital to optimize the performance of immobilized enzymes in micro/nanoscopic environments. In this Concept article, we review different analytical techniques that address single-particle studies to image the spatial distribution of the enzymes across the solid surfaces, the sub-particle substrate diffusion, the structural integrity and mobility of the immobilized enzymes inside the solid particles, and the pH and O2 internal gradients. From our view, such sub-particle information elicited from single-particle analysis is paramount for the design and fabrication of optimal heterogeneous biocatalyst.

KW - heterogeneous biocatalysis

KW - microscopy

KW - multi-enzymatic systems

KW - protein immobilization

KW - spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85040542338&partnerID=8YFLogxK

U2 - 10.1002/cctc.201701590

DO - 10.1002/cctc.201701590

M3 - Article

VL - 10

SP - 654

EP - 665

JO - ChemCatChem

JF - ChemCatChem

SN - 1867-3880

IS - 4

ER -