Signal enhancement in DNA microarray using dye doped silica nanoparticles: Application to Human Papilloma Virus (HPV) detection

Raffaele Riccò, Anna Meneghello, Francesco Enrichi

Research output: Contribution to journalArticleResearchpeer-review

Abstract

DNA microarray is a powerful tool for the parallel of nucleic acids and other biologically significant molecules. In this communication we report an easy and cheap synthesis route for incorporating organic dyes into monodisperse inorganic silica nanoparticles and their application on the detection of carcinogenic risky Human Papilloma Virus using DNA microarray technology. We correlate our system with conventional direct dyes and commercial quantum dots, with a promising increase in optical signal, and a related decrease of the limit of detection, thus giving a remarkable improvement in this technique towards early diagnosis of diseases and trace level detection of dangerous biological contaminants.

Original languageEnglish
Pages (from-to)2761-2765
Number of pages5
JournalBiosensors & Bioelectronics
Volume26
Issue number5
DOIs
Publication statusPublished - 15 Jan 2011
Externally publishedYes

Fingerprint

Papillomaviridae
Microarrays
Oligonucleotide Array Sequence Analysis
Viruses
Silicon Dioxide
Nanoparticles
DNA
Coloring Agents
Dyes
Silica
Quantum Dots
Nucleic acids
Nucleic Acids
Semiconductor quantum dots
Limit of Detection
Early Diagnosis
Impurities
Technology
Molecules
Communication

Keywords

  • DNA microarray
  • Fluorescence sensor
  • HPV detection
  • Silica nanoparticles

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Signal enhancement in DNA microarray using dye doped silica nanoparticles : Application to Human Papilloma Virus (HPV) detection. / Riccò, Raffaele; Meneghello, Anna; Enrichi, Francesco.

In: Biosensors & Bioelectronics, Vol. 26, No. 5, 15.01.2011, p. 2761-2765.

Research output: Contribution to journalArticleResearchpeer-review

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