Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1

Griet Dewitte, Maarten Walmagh, Margo Diricks, Alexander Lepak, Alexander Gutmann, Bernd Nidetzky, Tom Desmet

Research output: Contribution to journalArticleResearchpeer-review

Abstract

UDP-glycosyltransferases (UGTs) are a promising class of biocatalysts that offer a sustainable alternative for chemical glycosylation of natural products. In this study, we aimed to characterize plant-derived UGTs from the GT-1 family with an emphasis on their acceptor promiscuity and their potential application in glycosylation processes. Recombinant expression in E. coli provided sufficient amounts of enzyme for the in-depth characterization of the salicylic acid UGT from Capsella rubella (UGT-SACr) and the stevia UGT from Stevia rebaudiana (UGT-76G1Sr). The latter was found to have a remarkably broad specificity with activities on a wide diversity of structures, from aliphatic and branched alcohols, over small phenolics to larger flavonoids, terpenoids and even higher glycoside compounds. As an example for its industrial potential, the glycosylation of curcumin was thoroughly evaluated. Under optimized conditions, 96% of curcumin was converted within 24 h into the corresponding curcumin β-glycosides. In addition, the reaction was performed in a coupled system with sucrose synthase from Glycine max, to enable the cost-efficient (re)generation of UDP-Glc from sucrose as abundant and renewable resource.
LanguageEnglish
Pages49-55
JournalJournal of Biotechnology
Volume233
DOIs
StatusPublished - 1 Jun 2016

Fingerprint

Stevia
Glycosyltransferases
Uridine Diphosphate
Curcumin
Glycosylation
Glycosides
Capsella
Salicylic Acid
Rubella
Terpenes
Enzymes
Biological Products
Soybeans
Flavonoids
Sucrose
Alcohols
Escherichia coli
Costs and Cost Analysis

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)

Cite this

Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1. / Dewitte, Griet; Walmagh, Maarten; Diricks, Margo; Lepak, Alexander; Gutmann, Alexander; Nidetzky, Bernd; Desmet, Tom.

In: Journal of Biotechnology, Vol. 233, 01.06.2016, p. 49-55.

Research output: Contribution to journalArticleResearchpeer-review

Dewitte, Griet ; Walmagh, Maarten ; Diricks, Margo ; Lepak, Alexander ; Gutmann, Alexander ; Nidetzky, Bernd ; Desmet, Tom. / Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1. In: Journal of Biotechnology. 2016 ; Vol. 233. pp. 49-55
@article{370316f13dd14e3c85e54dd84511964c,
title = "Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1",
abstract = "UDP-glycosyltransferases (UGTs) are a promising class of biocatalysts that offer a sustainable alternative for chemical glycosylation of natural products. In this study, we aimed to characterize plant-derived UGTs from the GT-1 family with an emphasis on their acceptor promiscuity and their potential application in glycosylation processes. Recombinant expression in E. coli provided sufficient amounts of enzyme for the in-depth characterization of the salicylic acid UGT from Capsella rubella (UGT-SACr) and the stevia UGT from Stevia rebaudiana (UGT-76G1Sr). The latter was found to have a remarkably broad specificity with activities on a wide diversity of structures, from aliphatic and branched alcohols, over small phenolics to larger flavonoids, terpenoids and even higher glycoside compounds. As an example for its industrial potential, the glycosylation of curcumin was thoroughly evaluated. Under optimized conditions, 96{\%} of curcumin was converted within 24 h into the corresponding curcumin β-glycosides. In addition, the reaction was performed in a coupled system with sucrose synthase from Glycine max, to enable the cost-efficient (re)generation of UDP-Glc from sucrose as abundant and renewable resource.",
author = "Griet Dewitte and Maarten Walmagh and Margo Diricks and Alexander Lepak and Alexander Gutmann and Bernd Nidetzky and Tom Desmet",
year = "2016",
month = "6",
day = "1",
doi = "10.1016/j.jbiotec.2016.06.034",
language = "English",
volume = "233",
pages = "49--55",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1

AU - Dewitte,Griet

AU - Walmagh,Maarten

AU - Diricks,Margo

AU - Lepak,Alexander

AU - Gutmann,Alexander

AU - Nidetzky,Bernd

AU - Desmet,Tom

PY - 2016/6/1

Y1 - 2016/6/1

N2 - UDP-glycosyltransferases (UGTs) are a promising class of biocatalysts that offer a sustainable alternative for chemical glycosylation of natural products. In this study, we aimed to characterize plant-derived UGTs from the GT-1 family with an emphasis on their acceptor promiscuity and their potential application in glycosylation processes. Recombinant expression in E. coli provided sufficient amounts of enzyme for the in-depth characterization of the salicylic acid UGT from Capsella rubella (UGT-SACr) and the stevia UGT from Stevia rebaudiana (UGT-76G1Sr). The latter was found to have a remarkably broad specificity with activities on a wide diversity of structures, from aliphatic and branched alcohols, over small phenolics to larger flavonoids, terpenoids and even higher glycoside compounds. As an example for its industrial potential, the glycosylation of curcumin was thoroughly evaluated. Under optimized conditions, 96% of curcumin was converted within 24 h into the corresponding curcumin β-glycosides. In addition, the reaction was performed in a coupled system with sucrose synthase from Glycine max, to enable the cost-efficient (re)generation of UDP-Glc from sucrose as abundant and renewable resource.

AB - UDP-glycosyltransferases (UGTs) are a promising class of biocatalysts that offer a sustainable alternative for chemical glycosylation of natural products. In this study, we aimed to characterize plant-derived UGTs from the GT-1 family with an emphasis on their acceptor promiscuity and their potential application in glycosylation processes. Recombinant expression in E. coli provided sufficient amounts of enzyme for the in-depth characterization of the salicylic acid UGT from Capsella rubella (UGT-SACr) and the stevia UGT from Stevia rebaudiana (UGT-76G1Sr). The latter was found to have a remarkably broad specificity with activities on a wide diversity of structures, from aliphatic and branched alcohols, over small phenolics to larger flavonoids, terpenoids and even higher glycoside compounds. As an example for its industrial potential, the glycosylation of curcumin was thoroughly evaluated. Under optimized conditions, 96% of curcumin was converted within 24 h into the corresponding curcumin β-glycosides. In addition, the reaction was performed in a coupled system with sucrose synthase from Glycine max, to enable the cost-efficient (re)generation of UDP-Glc from sucrose as abundant and renewable resource.

U2 - 10.1016/j.jbiotec.2016.06.034

DO - 10.1016/j.jbiotec.2016.06.034

M3 - Article

VL - 233

SP - 49

EP - 55

JO - Journal of Biotechnology

T2 - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

ER -