Rapid separation and quantitative analysis of complex lipophilic wood pulp extractive mixtures based on 2D Thin Layer Chromotography

Werner Schlemmer, Melissa Sophie Egger, Madeline Dächert, Jussi Antero Lahti, Markus Gschiel, Andrea Walzl, Erich Leitner, Stefan Spirk*, Ulrich Hirn

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

We present a rapid method based on 2D thin layer chromatography (TLC) for the simultaneous separation and quantification of organic, lipophilic extractives occurring in lignocellulosic biorefineries. The separation is based on alkaline followed by acidic elution for a 2D separation of the different compound classes (sterols, fatty alcohols, fatty acids, and resin acids). Staining with Nile Blue A and subsequent scanning and image analysis of the plates provides quantitative information on the concentration of each compound class. Correlation coefficients between 0.92 (abietic acid, resin acid) and 0.98 (β-sitosterol, sterol) are determined in these experiments. Besides the analysis of the composition, this procedure indicates the relative polarity as the acidity or basicity of the constituents. TLC results of an industrial lipophilic extractives sample correlate with state-of-the-art GC × GC–MS measurements with differences being in the range between 0.5 (fatty alcohols) and 6% (resin acids), respectively.
Original languageEnglish
Pages (from-to)12534-12541
JournalACS Sustainable Chemistry & Engineering
Volume8
Issue number33
DOIs
Publication statusPublished - 2020

Keywords

  • Pitch
  • Resin
  • 2D TLC
  • Quantification
  • optical sensing

Fields of Expertise

  • Sustainable Systems

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    CD-Laboratory for Fiber Swelling and Paper Performance

    Hirn, U., Lahti, J. A., Schennach, E., Fischer, W. J., Czibula, C. M., Urstöger, G. J., Ganser, C., Schuller, M., Krainer, S., Xhori, R., Spirk, S. & Niegelhell, K.

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