Protein surface functionalisation as a general strategy for facilitating biomimetic mineralisation of ZIF-8

Natasha K. Maddigan, Andrew Tarzia, David M. Huang, Christopher J. Sumby, Stephen G. Bell*, Paolo Falcaro, Christian J. Doonan

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The durability of enzymes in harsh conditions can be enhanced by encapsulation within metal-organic frameworks (MOFs) via a process called biomimetic mineralisation. Herein we show that the surface charge and chemistry of a protein determines its ability to seed MOF growth. We demonstrate that chemical modification of amino acids on the protein surface is an effective method for systematically controlling biomimetic mineralisation by zeolitic imidazolate framework-8 (ZIF-8). Reaction of surface lysine residues with succinic (or acetic) anhydride facilitates biomimetic mineralisation by increasing the surface negative charge, whereas reaction of surface carboxylate moieties with ethylenediamine affords a more positively charged protein and hinders the process. Moreover, computational studies confirm that the surface electrostatic potential of a protein is a good indicator of its ability to induce biomimetic mineralisation. This study highlights the important role played by protein surface chemistry in encapsulation and outlines a general method for facilitating the biomimetic mineralisation of proteins.

Original languageEnglish
Pages (from-to)4217-4223
Number of pages7
JournalChemical Science
Volume9
Issue number18
DOIs
Publication statusPublished - 1 Jan 2018

ASJC Scopus subject areas

  • Chemistry(all)

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