Probing the enantioselectivity of Bacillus subtilis esterase BS2 for tert. alcohols

M Wiggers, J Holt, R Kourist, S Bartsch, Iwce Arends, A J Minnaard, U T Bornscheuer, U Hanefeld

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The activity and enantioselectivity of several mutants of the esterase BS2 from Bacillus subtilis have been investigated. In the enzymatic hydrolysis of alpha,alpha-disubstituted cyanohydrin acetates, a class of tert. alcohol esters, they were active but not selective. In contrast to this result similar tert. acetylenic alcohol esters were hydrolysed with high E-values (>100). The difference in reactivity has been studied by molecular dynamics studies. The computer model suggested that the source of the observed difference in reactivity between the two very similar tert. alcohol esters lies in the ability of the cyanohydrins to form hydrogen bonds to water molecules-even when the substrate is in the active site. (C) 2009 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)82-86
Number of pages5
JournalJournal of molecular catalysis / B
Volume60
DOIs
Publication statusPublished - 2009

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Enantioselectivity
Bacilli
Esterases
Bacillus subtilis
Esters
Alcohols
Enzymatic hydrolysis
Molecular Dynamics Simulation
Computer Simulation
Molecular dynamics
Hydrogen
Catalytic Domain
Hydrogen bonds
Acetates
Hydrolysis
Molecules
Water
Substrates
cyanohydrin

Keywords

  • Acetylenic alcohol Bacillus subtilis esterase BS2 candida-cylindracea Cyanohydrin cyanohydrin acetates enzyme Enzyme catalysis kinetic resolution lipase mutants tert. alcohol tertiary alcohols

Cite this

Probing the enantioselectivity of Bacillus subtilis esterase BS2 for tert. alcohols. / Wiggers, M; Holt, J; Kourist, R; Bartsch, S; Arends, Iwce; Minnaard, A J; Bornscheuer, U T; Hanefeld, U.

In: Journal of molecular catalysis / B, Vol. 60, 2009, p. 82-86.

Research output: Contribution to journalArticleResearchpeer-review

Wiggers, M, Holt, J, Kourist, R, Bartsch, S, Arends, I, Minnaard, AJ, Bornscheuer, UT & Hanefeld, U 2009, 'Probing the enantioselectivity of Bacillus subtilis esterase BS2 for tert. alcohols' Journal of molecular catalysis / B, vol. 60, pp. 82-86. https://doi.org/10.1016/j.molcatb.2009.04.003
Wiggers, M ; Holt, J ; Kourist, R ; Bartsch, S ; Arends, Iwce ; Minnaard, A J ; Bornscheuer, U T ; Hanefeld, U. / Probing the enantioselectivity of Bacillus subtilis esterase BS2 for tert. alcohols. In: Journal of molecular catalysis / B. 2009 ; Vol. 60. pp. 82-86.
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abstract = "The activity and enantioselectivity of several mutants of the esterase BS2 from Bacillus subtilis have been investigated. In the enzymatic hydrolysis of alpha,alpha-disubstituted cyanohydrin acetates, a class of tert. alcohol esters, they were active but not selective. In contrast to this result similar tert. acetylenic alcohol esters were hydrolysed with high E-values (>100). The difference in reactivity has been studied by molecular dynamics studies. The computer model suggested that the source of the observed difference in reactivity between the two very similar tert. alcohol esters lies in the ability of the cyanohydrins to form hydrogen bonds to water molecules-even when the substrate is in the active site. (C) 2009 Elsevier B.V. All rights reserved.",
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AU - Wiggers, M

AU - Holt, J

AU - Kourist, R

AU - Bartsch, S

AU - Arends, Iwce

AU - Minnaard, A J

AU - Bornscheuer, U T

AU - Hanefeld, U

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PY - 2009

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AB - The activity and enantioselectivity of several mutants of the esterase BS2 from Bacillus subtilis have been investigated. In the enzymatic hydrolysis of alpha,alpha-disubstituted cyanohydrin acetates, a class of tert. alcohol esters, they were active but not selective. In contrast to this result similar tert. acetylenic alcohol esters were hydrolysed with high E-values (>100). The difference in reactivity has been studied by molecular dynamics studies. The computer model suggested that the source of the observed difference in reactivity between the two very similar tert. alcohol esters lies in the ability of the cyanohydrins to form hydrogen bonds to water molecules-even when the substrate is in the active site. (C) 2009 Elsevier B.V. All rights reserved.

KW - Acetylenic alcohol Bacillus subtilis esterase BS2 candida-cylindracea Cyanohydrin cyanohydrin acetates enzyme Enzyme catalysis kinetic resolution lipase mutants tert. alcohol tertiary alcohols

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