@inbook{b6809cdc3e214ceda1f2dff1db43c258,
title = "Practical Considerions Regarding the Choice of High-Throughput-Assays",
abstract = "Since the first successful directed evolution studies in the 1990s, experience shows that the success of protein engineering for an enzyme of interest mainly depends on the extent of knowledge of its structure-function relationships and the ability to characterize a sufficiently large number of enzyme variants.1 A good understanding of a catalytic property makes it possible to achieve an objective with very few amino acid exchanges. In contrast, a powerful assay makes it possible to extend the number of investigated variants and thus to increase the likelihood to find an improved mutant. ",
author = "Robert Kourist and Carolin M{\"u}gge",
year = "2017",
month = sep,
day = "1",
language = "English",
isbn = "978-1-4939-7364-4",
volume = "1685",
series = "Springer Protocols Methods in Molecular Biology",
publisher = "Humana Press",
pages = "189--208",
editor = "Uwe Bornscheuer and Matthias H{\"o}hne",
booktitle = "Protein Engineering- Methods and Protocols",
}