Practical Considerions Regarding the Choice of High-Throughput-Assays

Robert Kourist, Carolin Mügge

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Since the first successful directed evolution studies in the 1990s, experience shows that the success of protein engineering for an enzyme of interest mainly depends on the extent of knowledge of its structure-function relationships and the ability to characterize a sufficiently large number of enzyme variants.1 A good understanding of a catalytic property makes it possible to achieve an objective with very few amino acid exchanges. In contrast, a powerful assay makes it possible to extend the number of investigated variants and thus to increase the likelihood to find an improved mutant.
LanguageEnglish
Title of host publicationProtein Engineering- Methods and Protocols
EditorsUwe Bornscheuer, Matthias Höhne
Pages189-208
Number of pages18
Volume1685
StatusPublished - 1 Sep 2017

Publication series

NameSpringer Protocols Methods in Molecular Biology
PublisherHumana Press

Fingerprint

Assays
Throughput
Enzymes
Amino Acids
Proteins

Fields of Expertise

  • Human- & Biotechnology

Cite this

Kourist, R., & Mügge, C. (2017). Practical Considerions Regarding the Choice of High-Throughput-Assays. In U. Bornscheuer, & M. Höhne (Eds.), Protein Engineering- Methods and Protocols (Vol. 1685, pp. 189-208). (Springer Protocols Methods in Molecular Biology).

Practical Considerions Regarding the Choice of High-Throughput-Assays. / Kourist, Robert; Mügge, Carolin.

Protein Engineering- Methods and Protocols. ed. / Uwe Bornscheuer; Matthias Höhne. Vol. 1685 2017. p. 189-208 (Springer Protocols Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kourist, R & Mügge, C 2017, Practical Considerions Regarding the Choice of High-Throughput-Assays. in U Bornscheuer & M Höhne (eds), Protein Engineering- Methods and Protocols. vol. 1685, Springer Protocols Methods in Molecular Biology, pp. 189-208.
Kourist R, Mügge C. Practical Considerions Regarding the Choice of High-Throughput-Assays. In Bornscheuer U, Höhne M, editors, Protein Engineering- Methods and Protocols. Vol. 1685. 2017. p. 189-208. (Springer Protocols Methods in Molecular Biology).
Kourist, Robert ; Mügge, Carolin. / Practical Considerions Regarding the Choice of High-Throughput-Assays. Protein Engineering- Methods and Protocols. editor / Uwe Bornscheuer ; Matthias Höhne. Vol. 1685 2017. pp. 189-208 (Springer Protocols Methods in Molecular Biology).
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