Oxygen Management at the Microscale: A Functional Biochip Material with Long-Lasting and Tunable Oxygen Scavenging Properties for Cell Culture Applications

Drago Sticker, Mario Rothbauer, Josef Ehgartner, Christoph Steininger, Olga Liske, Robert Liska, Winfried Neuhaus, Torsten Mayr, Tommy Haraldsson, Jörg P. Kutter, Peter Ertl*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Oxygen plays a pivotal role in cellular homeostasis, and its partial pressure determines cellular function and fate. Consequently, the ability to control oxygen tension is a critical parameter for recreating physiologically relevant in vitro culture conditions for mammalian cells and microorganisms. Despite its importance, most microdevices and organ-on-a-chip systems to date overlook oxygen gradient parameters because controlling oxygen often requires bulky and expensive external instrumental setups. To overcome this limitation, we have adapted an off-stoichiometric thiol-ene-epoxy polymer to efficiently remove dissolved oxygen to below 1 hPa and also integrated this modified polymer into a functional biochip material. The relevance of using an oxygen scavenging material in microfluidics is that it makes it feasible to readily control oxygen depletion rates inside the biochip by simply changing the surface-to-volume aspect ratio of the microfluidic channel network as well as by changing the temperature and curing times during the fabrication process.

Original languageEnglish
Pages (from-to)9730-9739
Number of pages10
JournalACS Applied Materials and Interfaces
Volume11
Issue number10
DOIs
Publication statusPublished - 13 Mar 2019

Keywords

  • bacteria
  • functional material
  • mammalian cells
  • oxygen control
  • oxygen scavenging
  • thiol-ene

ASJC Scopus subject areas

  • Materials Science(all)

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