Novel insights into plant-associated archaea and their functioning in arugula (Eruca sativa Mill.)

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A plant's microbiota has various implications for the plant's health and performance; however, the roles of many microbial lineages, particularly Archaea, have not been explored in detail. In the present study, analysis of archaea-specific 16S rRNA gene fragments and shotgun-sequenced metagenomes was combined with visualization techniques to obtain the first insights into the archaeome of a common salad plant, arugula (Eruca sativa Mill.). The archaeal communities associated with the soil, rhizosphere and phyllosphere were distinct, but a high proportion of community members were shared among all analysed habitats. Soil habitats exhibited the highest diversity of Archaea, followed by the rhizosphere and the phyllosphere. The archaeal community was dominated by Thaumarchaeota and Euryarchaeota, with the most abundant taxa assigned to Candidatus Nitrosocosmicus, species of the ‘Soil Crenarchaeotic Group’ and, interestingly, Methanosarcina. Moreover, a large number of archaea-assigned sequences remained unassigned at lower taxonomic levels. Overall, analysis of shotgun-sequenced total-community DNA revealed a more diverse archaeome. Differences were evident at the class level and at higher taxonomic resolutions when compared to results from the 16S rRNA gene fragment amplicon library. Functional assessments primarily revealed archaeal genes related to response to stress (especially oxidative stress), CO 2 fixation, and glycogen degradation. Microscopic visualizations of fluorescently labelled archaea in the phyllosphere revealed small scattered colonies, while archaea in the rhizosphere were found to be embedded within large bacterial biofilms. Altogether, Archaea were identified as a rather small but niche-specific component of the microbiomes of the widespread leafy green plant arugula.

LanguageEnglish
Number of pages10
JournalJournal of Advanced Research
DOIs
StatusE-pub ahead of print - 1 Jan 2019

Fingerprint

arugula
Eruca vesicaria subsp. sativa
Archaea
phyllosphere
rhizosphere
Methanosarcina
ribosomal RNA
Euryarchaeota
plant health
genes
salads
green leafy vegetables
biofilm
glycogen
soil
stress response
niches
oxidative stress
degradation

Keywords

  • Archaea
  • Brassicaceae
  • Eruca sativa Mill.
  • Holobiont
  • Metagenomics
  • Microbiome

ASJC Scopus subject areas

  • General

Cite this

Novel insights into plant-associated archaea and their functioning in arugula (Eruca sativa Mill.). / Taffner, Julian; Cernava, Tomislav; Erlacher, Armin; Berg, Gabriele.

In: Journal of Advanced Research, 01.01.2019.

Research output: Contribution to journalArticleResearchpeer-review

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