Novel electrospun fibers with incorporated commensal bacteria for potential preventive treatment of the diabetic foot

Manja Kurečič, Tomaž Rijavec, Silvo Hribernik, Aleš Lapanje, Karin S. Kleinschek, Uroš Maver

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Aim: A novel electrospun biocompatible nanofibrous material loaded with commensal bacteria for potential preventive treatment of the diabetic foot was developed. Materials & methods: Two biocompatible polymers (carboxymethylcellulose and polyethylene oxide) were combined with a bacterium isolate from the skin located between the toes of a healthy adult (identified using a matrix-Assisted laser desorption/ionization mass spectrometry-based method as a strain of Staphylococcus epidermidis). Higher bacteria loads in the material were assured through their encapsulation in polyethylenimine. The nanofibrous material was characterized using scanning electron microscopy, zeta-potential measurements and through evaluation of cell growth and viability. Results & discussion: nanometer formation was confirmed using scanning electron microscopy, while the zeta-potential measurements revealed successful bacteria encapsulation. Viable and sufficiently growing cells were confirmed prior and after their incorporation. Conclusion: The prepared materials were proven suitable to deliver viable commensal bacteria in a comparable share to the Staphylococcaceae in the foot microbiome making this approach promising for preventive diabetic foot treatment.

Original languageEnglish
Pages (from-to)1583-1594
Number of pages12
JournalNanomedicine
Volume13
Issue number13
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Diabetic Foot
Bacteria
Fibers
Zeta potential
Encapsulation
Staphylococcaceae
Electron Scanning Microscopy
Therapeutics
Polyethyleneimine
Carboxymethylcellulose Sodium
Scanning electron microscopy
Staphylococcus epidermidis
Microbiota
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Cell growth
Toes
Biocompatible Materials
Polyethylene oxides
Biomaterials
Ionization

Keywords

  • cell electrospinning
  • commensal bacteria
  • controlled release
  • diabetic foot
  • prophylactic/preventive treatment
  • release kinetics

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Novel electrospun fibers with incorporated commensal bacteria for potential preventive treatment of the diabetic foot. / Kurečič, Manja; Rijavec, Tomaž; Hribernik, Silvo; Lapanje, Aleš; Kleinschek, Karin S.; Maver, Uroš.

In: Nanomedicine, Vol. 13, No. 13, 01.01.2018, p. 1583-1594.

Research output: Contribution to journalArticleResearchpeer-review

Kurečič, Manja ; Rijavec, Tomaž ; Hribernik, Silvo ; Lapanje, Aleš ; Kleinschek, Karin S. ; Maver, Uroš. / Novel electrospun fibers with incorporated commensal bacteria for potential preventive treatment of the diabetic foot. In: Nanomedicine. 2018 ; Vol. 13, No. 13. pp. 1583-1594.
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