TY - JOUR
T1 - Non-canonical amino acids as a useful synthetic biological tool for lipase-catalysed reactions in hostile environments
AU - Acevedo-Rocha, Carlos G.
AU - Hoesl, Michael G.
AU - Nehring, Sebastian
AU - Royter, Marina
AU - Wolschner, Christina
AU - Wiltschi, Birgit
AU - Antranikian, Garabed
AU - Budisa, Nediljko
PY - 2013/5/1
Y1 - 2013/5/1
N2 - The incorporation of several non-canonical amino acids into the Thermoanaerobacter thermohydrosulfuricus lipase confers not only activity enhancement upon treatment with organic solvents (by up to 450%) and surfactants (resp. 1630%), but also protective effects against protein reducing (resp. 140%), alkylating (resp. 160%), and denaturing (resp.190%) agents as well as inhibitors (resp. 40%). This approach offers novel chemically diversified biocatalysts for hostile environments.
AB - The incorporation of several non-canonical amino acids into the Thermoanaerobacter thermohydrosulfuricus lipase confers not only activity enhancement upon treatment with organic solvents (by up to 450%) and surfactants (resp. 1630%), but also protective effects against protein reducing (resp. 140%), alkylating (resp. 160%), and denaturing (resp.190%) agents as well as inhibitors (resp. 40%). This approach offers novel chemically diversified biocatalysts for hostile environments.
UR - http://www.scopus.com/inward/record.url?scp=84879579137&partnerID=8YFLogxK
U2 - 10.1039/c3cy20712a
DO - 10.1039/c3cy20712a
M3 - Article
AN - SCOPUS:84879579137
SN - 2044-4753
VL - 3
SP - 1198
EP - 1201
JO - Catalysis Science and Technology
JF - Catalysis Science and Technology
IS - 5
ER -