Myeloperoxidase-responsive materials for infection detection based on immobilized aminomethoxyphenol

Doris Schiffer, Gregor Tegl, Robert Vielnascher, Hansjörg Weber, Alexandra Herrero-Rollett, Eva Maria Sigl, Andrea Heinzle, Georg M. Guebitz

Research output: Contribution to journalArticleResearchpeer-review

Abstract

There is a strong need for simple and fast diagnostic tools for the detection of wound infection. Immune system-derived enzymes like myeloperoxidase are efficient biomarkers for wound infection that emerge in the early stage infection process. In this study, 5-amino-2-methoxyphenol was functionalized with alkoxysilane to allow visual detection of MPO on carrier materials, for example, in test strips. Indeed, MPO activity was visually detectable in short time in wound background. Oxidation of the substrate was followed spectrophotometrically and proved via HPLC. LC-ESI TOF and NMR analyses unveiled the reaction mechanism and a dimeric reaction product responsible for the visualization of MPO activity. The substrate specificity and sensitivity toward MPO detection was proved and tests with infected wound fluids were successfully performed. The study demonstrates the suitability of the novel MPO substrate for the detection of wound infection and the covalent immobilization on diagnostic carrier materials.

Original languageEnglish
Pages (from-to)2553-2560
JournalBiotechnology and Bioengineering
Volume113
Issue number12
DOIs
Publication statusPublished - 2016

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Keywords

  • Biomarker
  • Chronic wounds
  • Color change
  • Diagnostics
  • Enzymes
  • Immobilization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Schiffer, D., Tegl, G., Vielnascher, R., Weber, H., Herrero-Rollett, A., Sigl, E. M., ... Guebitz, G. M. (2016). Myeloperoxidase-responsive materials for infection detection based on immobilized aminomethoxyphenol. Biotechnology and Bioengineering, 113(12), 2553-2560. https://doi.org/10.1002/bit.26025