Molecular cloning and functional characterization of a two highly stereoselective borneol dehydrogenases from Salvia officinalis L

Ivana Drienovská, Dajana Kolanović, Andrea Chánique, Volker Sieber, Michael Hofer, Robert Kourist*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Enzymes for selective terpene functionalization are of particular importance for industrial applications. Pure enantiomers of borneol and isoborneol are fragrant constituents of several essential oils and find frequent application in cosmetics and therapy. Racemic borneol can be easily obtained from racemic camphor, which in turn is readily available from industrial side-streams. Enantioselective biocatalysts for the selective conversion of borneol and isoborneol stereoisomers would be therefore highly desirable for their catalytic separation under mild reaction conditions. Although several borneol dehydrogenases from plants and bacteria have been reported, none show sufficient stereoselectivity. Despite Croteau et al. describing sage leaves to specifically oxidize one borneol enantiomer in the late 70s, no specific enzymes have been characterized. We expected that one or several alcohol dehydrogenases encoded in the recently elucidated genome of Salvia officinalis L. would, therefore, be stereoselective. This study thus reports the recombinant expression in E. coli and characterization of two enantiospecific enzymes from the Salvia officinalis L. genome, SoBDH1 and SoBDH2, and their comparison to other known ADHs. Both enzymes produce preferentially (+)-camphor from racemic borneol, but (−)-camphor from racemic isoborneol.

Original languageEnglish
Article number112227
JournalPhytochemistry
Volume172
DOIs
Publication statusPublished - Apr 2020

Keywords

  • Borneol
  • Borneol dehydrogenases
  • Camphor
  • Lamiaceae
  • Monoterpenes
  • Salvia officinalis L.
  • Selective oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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