Modified Stöber synthesis of highly luminescent dye-doped silica nanoparticles

G. Canton, R. Ricco, F. Marinello, S. Carmignato, F. Enrichi*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Dye-doped silica nanoparticles (NPs) have been demonstrated to be sensitive labeling markers for biosensing and bioimaging. Their flexible conjugation, excellent photostability and ultrasensitivity make them a powerful tool in biological analysis. Although there have been many reports on the basic research and application of these NPs, they are far from reaching their full potential. Silica NPs can be obtained through two principal approaches: the Stöber synthesis and the microemulsion method. The Stöber synthesis has the advantage of being easily scaled up for commercial applications and the possibility to effortlessly transfer the NPs into aqueous solutions (typical of bioanalysis). However, further investigation on the impact of the synthesis parameters on the particles size and on the doping process are needed in order to obtain highly luminescent particles. In this study a modified Stöber synthesis is proposed and a systematic study of the different reagents is reported, which provides a better picture on the influence of ethanol, ammonia, water, silica precursors, and dye concentration on the final morpho-optical properties. As a result, samples of luminescent silica NPs from 10 to 300 nm have been synthesized and optimized to be highly promising labels for biological applications.

Original languageEnglish
Pages (from-to)4349-4356
Number of pages8
JournalJournal of Nanoparticle Research
Volume13
Issue number9
DOIs
Publication statusPublished - Sep 2011
Externally publishedYes

Keywords

  • Biolabels
  • Biomarkers
  • Luminescent markers
  • Silica nanoparticles

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Modelling and Simulation
  • Chemistry(all)
  • Materials Science(all)
  • Bioengineering

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